FastQCFastQC Report
Wed 8 May 2024
EGAF00008269562

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008269562
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2938377
Total Bases146.9 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT131041844.596659992914454TruSeq Adapter, Index 19 (97% over 38bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG514681.7515791881028202TruSeq Adapter, Index 19 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTA85500.29097695768786647TruSeq Adapter, Index 19 (97% over 38bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT53140.1808481348717336TruSeq Adapter, Index 12 (97% over 35bp)
GATGGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT39830.13555102017202014TruSeq Adapter, Index 12 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)