FastQCFastQC Report
Wed 8 May 2024
EGAF00008269569

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008269569
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2322663
Total Bases116.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT855783.684477687895317TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG50900.219145007261062TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)