FastQCFastQC Report
Wed 8 May 2024
EGAF00008269888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008269888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2143119
Total Bases107.1 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTATG88520.4130428594959029TruSeq Adapter, Index 13 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT56890.26545422815998554TruSeq Adapter, Index 13 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)