FastQCFastQC Report
Thu 9 May 2024
EGAF00008269974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008269974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2234401
Total Bases279.3 Mbp
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCT90960.4070889692584277Illumina Single End Sequencing Primer (100% over 32bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATCTC70630.31610261542131424Illumina Single End Sequencing Primer (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)