FastQCFastQC Report
Wed 8 May 2024
EGAF00008270045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008270045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2014591
Total Bases251.8 Mbp
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTATG77490.38464383093143967TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTCGTAT65530.32527694206913466TruSeq Adapter, Index 19 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)