FastQCFastQC Report
Wed 8 May 2024
EGAF00008270100

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008270100
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2056739
Total Bases257 Mbp
Sequences flagged as poor quality0
Sequence length125
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCT23290.11323750850253728Illumina Single End PCR Primer 1 (97% over 34bp)
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGATAGGGTGTAGATCTC20820.10122820639857562Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)