FastQCFastQC Report
Wed 8 May 2024
EGAF00008270564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008270564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105306
Total Bases105.2 Mbp
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATG39000.18524623023921463TruSeq Adapter, Index 7 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT30540.14506204798732347TruSeq Adapter, Index 7 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)