FastQCFastQC Report
Sat 11 May 2024
EGAF00008272921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008272921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences163730946
Total Bases1.3 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC56

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTGCGC6715554041.015789403672045No Hit
GAGGATCT4075366224.890628800251356No Hit
CTTATGAG3242714019.80513811970524No Hit
AGACCATA1691495410.330945012679521No Hit
CTTAGGAG44613202.724787286088239No Hit
CTTCTGAG5240330.3200573946479244No Hit
AGACTATA2054250.12546498082286778No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)