FastQCFastQC Report
Fri 17 May 2024
EGAF00008272950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008272950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences247128112
Total Bases22.4 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT98229993.9748610226909356Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19975540.8083070694927659Clontech SMART CDS Primer II A (100% over 24bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9389730.3799539406508313Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7951290.3217476933583339No Hit
CAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3341160.1351995114177864Clontech SMART CDS Primer II A (100% over 23bp)
CCGGAATCGAACCCTGATTCCCCGTCACCCGTGGTCACCATGGTAGGCAC2860560.11575210836394041No Hit
AGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2856100.11557163516872576Clontech SMART CDS Primer II A (100% over 22bp)
ATCGAAAGTTGATAGGGCAGACGTTCGAATGGGTCGTCGCCGCCACGGGG2837880.11483436574791621No Hit
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC2586320.10465503010033922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)