FastQCFastQC Report
Fri 17 May 2024
EGAF00008272974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008272974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199876177
Total Bases18.1 Gbp
Sequences flagged as poor quality0
Sequence length91
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39193631.9608955198297595Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT7884130.39445071035153934Clontech SMART CDS Primer II A (100% over 24bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6469100.3236553799005271No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT3721600.18619527628847934Clontech SMART CDS Primer II A (100% over 25bp)
GTTATTTTTCGTCACTACCTCCCCGGGTCGGGAGTGGGTAATTTGCGCGC2210130.11057495861550323No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)