FastQCFastQC Report
Wed 8 May 2024
EGAF00008272996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008272996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28315370
Total Bases226.5 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGATAC937466633.10804697236872No Hit
ACGCGGGT777636627.463409448649266No Hit
GAATTCCA565655919.976991294833866Illumina Small RNA Adapter 2 (100% over 8bp)
TTTACATG521506118.417774516102032No Hit
CGCGATAA299470.10576234744592777No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)