FastQCFastQC Report
Wed 8 May 2024
EGAF00008272999

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008272999
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28963668
Total Bases231.7 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGATAC958834533.10473314360598No Hit
ACGCGGGT797079327.51997088214103No Hit
GAATTCCA578240619.964342914025945Illumina Small RNA Adapter 2 (100% over 8bp)
TTTACATG534211518.444193601445782No Hit
CGCGATAA304640.10518004832813302No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)