FastQCFastQC Report
Sat 11 May 2024
EGAF00008273002

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008273002
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230955050
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT6907531029.908551469214462No Hit
CTATAGTG5587132624.191428591840708Clontech Universal Primer Mix Short (100% over 8bp)
AGTCCCAC5583521624.17579351479866No Hit
TACGTTCA4872322621.096410751789147No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)