FastQCFastQC Report
Sat 11 May 2024
EGAF00008273011

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008273011
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228850275
Total Bases1.8 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGAGAGT6852472129.943036336748996No Hit
AGTCCCAC5531112624.169132416380098No Hit
CTATAGTG5530057524.16452197839832Clontech Universal Primer Mix Short (100% over 8bp)
TACGTTCA4820680121.064777396487724No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)