FastQCFastQC Report
Sat 11 May 2024
EGAF00008273020

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008273020
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196281262
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCCTT5390697127.46414530389559No Hit
CAGATGGG5157366126.27538689862306No Hit
AGTGGAAC4630387323.590572288046527No Hit
TCACATCA4280281521.806877826167632Illumina PCR Primer Index 1 (100% over 8bp)
GTCTCCTA4463650.22741090792456797No Hit
CAGATGGA2982120.15193095711805643No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)