FastQCFastQC Report
Sat 11 May 2024
EGAF00008273029

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008273029
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194587809
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length8
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTCCTT5358470027.537542190014584No Hit
CAGATGGG5103118426.22527293063873No Hit
AGTGGAAC4585873723.567117197974103No Hit
TCACATCA4235152521.764737070450288Illumina PCR Primer Index 1 (100% over 8bp)
GTCTCCTA4396910.22596019877072568No Hit
CAGATGGA2954460.151831711101696No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)