FastQCFastQC Report
Wed 8 May 2024
EGAF00008273050

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008273050
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24082970
Total Bases192.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCGAGAT829121634.42771385755162No Hit
TAATGACC586038524.334145663927664No Hit
ATGCCTTA483037720.057231313247495No Hit
CGTATCGG482574120.037981195840878No Hit
ATGCCTAA242740.10079321611910824Illumina PCR Primer Index 3 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)