FastQCFastQC Report
Tue 7 May 2024
EGAF00008325310

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008325310
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7155
Total Bases536.6 kbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT4015.604472396925227TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG440.6149545772187281No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC330.46121593291404617No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAGCTCGTAT140.1956673654786862TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG120.16771488469601675TruSeq Adapter, Index 3 (97% over 35bp)
CGCACAAATCTTCTCACAGGTGTCATTTTTCTTCTCCAATTAGCCATGTG110.15373864430468204No Hit
GCTGTTCTAGTTCGGCTTTCTCATGCTGCAACTGCATCAATTTTCTGGAG100.1397624039133473No Hit
AGCTGCTCGATGTGCATATTCTCCAGGAGCGCGCTGTGGGGCCGTAGGAC100.1397624039133473No Hit
CTAAATTCAATTACTAGCTTATTCTAAGTACTCCAGTTAATAAACGTTTG100.1397624039133473No Hit
GCCACTAAAATTACACGTTAAAAAAGAAAGTACATCACCCTTCCCGTGGT100.1397624039133473No Hit
ACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA100.1397624039133473No Hit
CTGTACATACATTTGTTCAAACACATTGAATTCTATACTTAAAGTGGGCT90.12578616352201258No Hit
CACCATGCGGTTCATACAATCTTGTTATTACTGTTAATTTATCAACTAAT90.12578616352201258No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG90.12578616352201258No Hit
ATCTGAGTCAGCAGGGATTTCCAATATATATTTTAAATGATAGAACACAG90.12578616352201258No Hit
GCACCGGTCATTTTTTGAGAACTGATTTGGTTTGTCCAACAAAGTAAACA90.12578616352201258No Hit
CCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGCTCAAGGCT90.12578616352201258No Hit
GCCAGGTGTTGACAACTATTTTGGGTGATGGTGAGTATATGGAGGGACGG90.12578616352201258No Hit
GTCATCTATTCACAAAACTGAAATACTTCATTACCCCAACTAAACATACA90.12578616352201258No Hit
TGGACATTGAAATTCTACTGTCCAATCCCAACTCACTGCTGTTGATTGGA90.12578616352201258No Hit
GGAGGCCTGGATGGCTCCCTTCTACACGGGGACTTTGAATTCCACTGCAG90.12578616352201258No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG90.12578616352201258No Hit
GGCTGCTTTCTGTTTCTGGGGTTCTCATTGCCTTTTACTATATTTACTCT90.12578616352201258No Hit
GCAAAGGCACTGAGCATAAAAATACATAAAGTGCATTACCAATGAATAGT90.12578616352201258No Hit
GTTACATTATAGCTTTTGCAGATAAGCCTGGGCATGTAACCATGTAACAT90.12578616352201258No Hit
CTTGTTGCAGATGAGAGAACTAAGGCTCCAGAGACTAAGCCAGGAAGCGC90.12578616352201258No Hit
GCCCTATTTTCTTTTCTGACACCTGTGATGAAGTTTTCACTTGGCCAATA90.12578616352201258No Hit
GGCCTGCTCAAATATGGAATAGTGATGTGGTCTCGGTTTGTCACTGAAGC80.11180992313067784No Hit
CTTCTAGTTCAAGTATTTTGAGGCTTGGTACCCAATGCTGATTTGTCATC80.11180992313067784No Hit
CATTGTTGTACAGCGGGGAGCCCAGCTCGGCTCTGAAGGTCTGCCGCATG80.11180992313067784No Hit
ACAAAATCAGTTATCTGCCAGCACGGACAAGCTGGAGAAGTGGTTCCCAA80.11180992313067784No Hit
CTTTCATGGGGAAGCAGATTTGGGTACCACCCAAGTATTGACTCACCCAT80.11180992313067784No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATATCGTAT80.11180992313067784TruSeq Adapter, Index 3 (97% over 36bp)
CCCTGTGTCCATGGAACATATTAATTTTTCTAATAGGATATTAATTGTAC80.11180992313067784No Hit
ACTCCACTTAACATAAAATCAGGAAAAAACAGTAAGTAAGATGTAAGGTA80.11180992313067784No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA80.11180992313067784No Hit
GTGCAGTGACAAGCAAAATTTTTGCTCTCCAATTTCTGAAAGTTATATGA80.11180992313067784No Hit
CGAACAATCATAGACATGTCCTATTTTTAGCAGGAGCTCAAAGAAAATGT80.11180992313067784No Hit
GATGCAGTTATGCCAAATCTGGTATTTTTGTTTTCTGAGAGAGAAGCCAG80.11180992313067784No Hit
GCTGCGGAGAAAGCTCTATGCTGAGAACATTCCCCTTGATGGAAAGAAGG80.11180992313067784No Hit
GCACCACTTAACAGTTGGAATACAGAAAGCTATTGATCCTATTGCACAAA80.11180992313067784No Hit
CTTCAGAAGCTTTCCTCTAATCATCAGGAAATATTCACCATCTTCACTGG80.11180992313067784No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT80.11180992313067784No Hit
GAAAAGGGACAGTAATTTGACACCGCAAATGTCAACGTGCACATGCCTGA80.11180992313067784No Hit
GTCATAAATAATGGAAAACTCCAAACACCAGTCATGAAAAGTAAACTTAA80.11180992313067784No Hit
CTCCTATTCCATCTCCCTGCTCCAAAAATCCATTTAATATATTGTCCTCG80.11180992313067784No Hit
CCAGGACCTGGTCCCAATCTGCTCATTTAACCTCACTTCCTATCACTTCA80.11180992313067784No Hit
CCTGTCTTTTCAGCCACCCGGGTGAGCAAAAGACAGAACAATGGCTCAGG80.11180992313067784No Hit
GGCCAACATGGTGACACCCAGTCTCCACTAAAAATATAAAAACTAGCCAA80.11180992313067784No Hit
GTCCAGGCTGAGCCACAGACTTGCTGTGACTTGGGGACTGCAATGAGCCC80.11180992313067784No Hit
AAGGAAGATTGCATCAATTGCCTCCTGCAGGGCTTGTACCACTTGAGGTT80.11180992313067784No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC80.11180992313067784No Hit
GCCACTATAGCTTTCTTTCACCTCATTTTTTAAATGTCGATCTTCACTTT80.11180992313067784No Hit
CTTATACTGATTTTCCATGAATGCGATTGTTTTAATAGTAACCTTTGCCA80.11180992313067784No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)