FastQCFastQC Report
Tue 7 May 2024
EGAF00008325316

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008325316
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6998
Total Bases524.8 kbp
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT3685.258645327236353TruSeq Adapter, Index 3 (97% over 36bp)
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC270.3858245212917976No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG220.3143755358673907No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAGCTCGTAT150.21434695627322092TruSeq Adapter, Index 3 (97% over 36bp)
CTCAGATCTCATAATCTTACCCTTCACTGAGAGGGACTTAACAATCTCGA130.18576736210345815No Hit
GGCATGGATATCTTTGGAGTCTTAAAATGCATCTCTGGCATCTTAAACTT120.17147756501857675No Hit
TCCTTTAGCTCCTTTACACTGTTTATATCTGCCTAGAAACTTCCAGCCCA110.15718776793369535No Hit
CCCAGGCGGTCAACTTAATGCGTTAGCTGCGCCACTAAGAGCTCAAGGCT110.15718776793369535No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA100.14289797084881395No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATATCGTAT100.14289797084881395TruSeq Adapter, Index 3 (97% over 36bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG100.14289797084881395TruSeq Adapter, Index 3 (97% over 35bp)
GTCTGGTCTGTGCCGGTGGATCAGGGCATTGAAGGCAAGACCATCCTTCC100.14289797084881395No Hit
CTCAGAATTTATATTTCCTAATTACAATTGATTTCCACAAAATTCAATGG100.14289797084881395No Hit
GGCCTCCATCCTCCTAACTTGAGAGGATGTGAATCATTCCACAGATGTCT90.12860817376393255No Hit
CAGTAATTAGAGCTAGAATCGAACCTGCCCTTGGAATATGTTTCTTTTTA90.12860817376393255No Hit
GTTTTATTAAAATTCTTTCTTGGGAATGTCATGACAATCTTTTGGCTAAA90.12860817376393255No Hit
CTCAGGGCCAGTTTATAAATACTCCATTCAGTACTATCTTAACACAAATC90.12860817376393255No Hit
CCCCTCCACCTTGCCTCAAGCTACTAAAACTCCCAGGGTGTTACCCAATA90.12860817376393255No Hit
CTCACTGTTTGGAAAAACACATAGAAACCAAGTCTGCGATGGCACTGGTA90.12860817376393255No Hit
ATGGGTTTAAGAACATTATTGTGCTGAATACTCTGCCTGATGGTTTCTAC90.12860817376393255No Hit
ACCACCACTAATTTCCCATCTTTCAATTTTCTTGTTATTGTGCTTTCCTT80.11431837667905116No Hit
CCTTACTTTCCTGCCCAAAGCATCCATTCCTGTTTCTTCCTCCAAGGCTT80.11431837667905116No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATGTCGTAT80.11431837667905116TruSeq Adapter, Index 3 (97% over 36bp)
GATTAGTTCTCATCCACATTGACTGTCTGTAGATCTGTAAAGGAAGTAAC80.11431837667905116No Hit
TTCGGCGAGGGAGTCTCTCACTCCCTTTATCGTTACTCATGTCAACATTC80.11431837667905116No Hit
GTCCTAGTGAAAAGCACATTAAGCATTTAACCTTGGAAAATCTTCTGCCT80.11431837667905116No Hit
CTGGCCTTTAACATTTTCAACAATTTTTACTGGGTTTAGTACATCAGTCA80.11431837667905116No Hit
CTTAGCCTCCCGAGTAGCAGGGACCACAGGCGTGCGCTGCTATGACTGGC80.11431837667905116No Hit
CTGGCCCCTACATTGCAGATGAGGCTCCCAATGTTGACGTTGGCCAGGGC80.11431837667905116No Hit
GCTGCGGAGAAAGCTCTATGCTGAGAACATTCCCCTTGATGGAAAGAAGG80.11431837667905116No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC80.11431837667905116No Hit
GGAATGTACTGATGCATGCTACAACATAGATGAATCTTGAAGACATTATG80.11431837667905116No Hit
CCTGTCTTTGGCAGCCTTCCACAATGATGAGCTTCTGCTGAGGGGAGGTC80.11431837667905116No Hit
GGCAATTTTCCATAAAAACAAAGGAGACTATGGAGACTGTCTGGTTCCTG80.11431837667905116No Hit
GTGGGTGCAGTCTAGTTCAAGTGTAACCTAGACTAAGAATGTGCAATCTT80.11431837667905116No Hit
GGCAAATTGGAATTCATAGAAAAGGATAAGACACTTCACACTACGTTGAA80.11431837667905116No Hit
GTGCTTGATGACAGAGACAGATTCACCTGTGAGAATTGACTGGTCAACTC80.11431837667905116No Hit
CCCGTCTGGTCCCATAGCGACTGGCTGTACTGTCCGTGTTGTCACAGTCT80.11431837667905116No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA80.11431837667905116No Hit
TGGGAAACCTAACTAGGGAGACTATTCTCTGGTGAAAGGTAAGCAAGTTT80.11431837667905116No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA70.10002857959416976No Hit
GCTGTTCTAGTTCGGCTTTCTCATGCTGCAACTGCATCAATTTTCTGGAG70.10002857959416976No Hit
CTGCAAAGTATCACTGGTTTGGTTCACAACAAGTACATCCAGGACAATAT70.10002857959416976No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC70.10002857959416976No Hit
GGGGAAGGGAAGGGAAACCTCTTGAGGTCCAAAGTTGCAAACAAAAAATG70.10002857959416976No Hit
CCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTTCACACGGCAG70.10002857959416976No Hit
CCATTTCATCCCCACTTCCAATCCCCAACATTTATACCCCGTGAATCAAT70.10002857959416976No Hit
GTAGAGATGGGGTTTGGGCATGTTTCCCAGGTTGATCTTGAACTCCTGGG70.10002857959416976No Hit
GCCTGGAAGACCTCCTCCGACAGTGGGGCCTTTGATCTCACCAGTGGCCC70.10002857959416976No Hit
ACTTGTTTCCTTGCTGATCTTTGATTGAAGGTTATTCTTACCTTCTAGAG70.10002857959416976No Hit
TGAATATGAAAAGTGTGGACTCTAGCCCATACTGCCAGTGCCTGATTCAT70.10002857959416976No Hit
CAATCACATCTTTGCCAATCTCTGTCACTGTGGTGACCCTGCAGATTTGA70.10002857959416976No Hit
GCCAGTATCAAAGAAGGGTCCATGTGATAAAAGAAGTGAAAAGCAGTCTT70.10002857959416976No Hit
CACTACAGTGCTTAGCAAGAGCTTTGGAACACAGGAAGCGTTTATTACAT70.10002857959416976No Hit
GGTTTTTTTGATTTGTTGTAGTTGATTGACTGTGTCCTTTACAAAGACTT70.10002857959416976No Hit
TTTTTTTCTTTAGTTTTCTGTTTGCTATTAGTTGGTTTTTTTAGATTCTA70.10002857959416976No Hit
CTTTAACTTAAGGTGCTATGTTTGAGGCACCATTGACTATGATTTGGGGT70.10002857959416976No Hit
TCCACATTGACTGTCTGTAGATTTTTGAAAGTGGTAACAGGTACATAGGT70.10002857959416976No Hit
CTCCTTTTCAACTTCAATGAGGTCAATCATAAGACTTCCTAAAAAGTCAT70.10002857959416976No Hit
CTTTTGACAAAAGGTGAAGAATAACTTTCAGAAGAGAAGTAGAAGTTCCA70.10002857959416976No Hit
CTAGGTTAAACTGTTAGAGAAAGGGATATGTGAACACATTTTTAAATTAA70.10002857959416976No Hit
GTGCTCATGGTGACACCGCACAGGACTTCCTGCCTGCTAGAAATCATCTA70.10002857959416976No Hit
GTCTGAGTGAGTTCTTCAGGGAAGCGATACTCTGAGTACCACAGGGACCA70.10002857959416976No Hit
GCCAAAGTTGTAATCTAGTAAGTCAAACTGCTTATCTCAATTAGGGGTAA70.10002857959416976No Hit
CTTTAGACATCATAACTAACATAATCGAGACGTGTTTACATTCCTACCAG70.10002857959416976No Hit
GTCATCAAGTAGCTGTTTTGTGAGCCCACATTTCATTGCAGCTGCAAATC70.10002857959416976No Hit
CCTGTGAGTTTCTCATTAGTGATTATTATATTGCAGGCAGCCAAAATGGA70.10002857959416976No Hit
GCCTTTTCCTTACCCAAAGCCTGATCTTATTAAACCTCAACTTATAATAT70.10002857959416976No Hit
GGGTTGATTCGTCAAAGATACCACTTCCTTTACCCCCTGGGGGATTTGTC70.10002857959416976No Hit
AGAATGATATTTCCTAGTCACCTAAATTGCTTGTGCATGATAGGCATTAT70.10002857959416976No Hit
CTATATTATTAAAACCAGAACCAGTTCACATCTGGAATTCCAATAGCTTT70.10002857959416976No Hit
ATTCCACTAATACTCGCCTATGATGCCAAACTTGATAGTTTTTGGGCTGC70.10002857959416976No Hit
CACTGGAATGCCTTGCATCAAATCTATAACAGCTTGCCTATAAGCATCCT70.10002857959416976No Hit
CCTTGATCTAGTGCTGGTCAAGTCTCTCCCCATGTCCCCACATTCAAATC70.10002857959416976No Hit
CTGAGACTAAATTTACAACAGCATGTTTGTCATTCAGCTTGAGAGCTGGA70.10002857959416976No Hit
GTGGCTTATCAACTAGGGGTCCTTCATACTAAAGCAAACCAAGTCTTTAC70.10002857959416976No Hit
CAGGGTCTCCGGAGTTTGGGCTTTTCATCTCCTTCACTTTCAGCTTCAGA70.10002857959416976No Hit
AGAAACTATCAGAGGAAAATTGCACATGGAACAGCTGGCTCTCGAGCAGG70.10002857959416976No Hit
GTCTGTGCCATAAGGTTAATTTCCTGATAATTTTTATCTGCATTAAAAAA70.10002857959416976No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG70.10002857959416976No Hit
ATTCCTTCTGAAGGTTTTACGATGCATTGTTATCATTAACCAGTCTTTTA70.10002857959416976No Hit
CTTACTCCTATCCCTATCTCTGTGCTCCAGCAAAGAGGTAGTTCCACTTA70.10002857959416976No Hit
GTCCTGCACCTCACACTCCACGTCCAGCACCTCACTCTCCGCTGACAGGT70.10002857959416976No Hit
CTTTAAACTGTCTAATTCGGTTTCTTTTTCAATTGCTTTATGTGTTACTG70.10002857959416976No Hit
CAATATAGTTATTTGGGGAAAGCAGGAAGATAGGATGGAGTGAGGGTAGG70.10002857959416976No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)