FastQCFastQC Report
Tue 7 May 2024
EGAF00008325327

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008325327
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6923
Total Bases519.2 kbp
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT3525.084500938899321TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG440.6355626173624151No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC230.33222591362126247No Hit
CTCAGATCTCATAATCTTACCCTTCACTGAGAGGGACTTAACAATCTCGA160.23111367904087823No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTATG160.23111367904087823TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAGCTCGTAT140.20222446916076847TruSeq Adapter, Index 3 (97% over 36bp)
CTTCACCATCAGCATACCCAAATGAGAGGCTGATTCCTCCATAGTTTCAA110.1588906543406038No Hit
CCAAGTTCAAAACATCACCCAATACCATGTATTTTTATCTACTCCCTCCA110.1588906543406038No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG100.1444460494005489No Hit
CTCCGTCAACTTCTGTCTTCTCTCCTGGTGAATAATCATATGTTTTAGAA100.1444460494005489No Hit
CTCTAGGAGCTTCTGAACTATGAGTTCAAATGAAGAAGATAAGCAGTAAG100.1444460494005489No Hit
CCCACAGTCATCACAAACATGAGGAAAAATTTTTGTATGTTCAATAGCAA100.1444460494005489No Hit
GTCCACAATAATAATACACAATATTATGAGCCTAATACAACAAAGTTGTC100.1444460494005489No Hit
GTTCTCAATAAATGTTTATTCCTTTTCCTTTCTGCTGCATTTCAAGAGGT90.130001444460494No Hit
CTCTGGCTTTGGTCTGAGTGAGACTGGAGACCACTGGAGGGTTTGGGACA90.130001444460494No Hit
GTACACACTGTTTCTGTAAAGTGACATCTTTCAGATACTTCGTGGCTTTT90.130001444460494No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG90.130001444460494No Hit
GGAACACGTGCTGCTAACTCACTGGTGAGTTCAATGGCAACGCTTCATTC90.130001444460494No Hit
GCCTGCAATGGGCTCATCAGTACACAGAACACAGTTAAGGAAAAAAAATC90.130001444460494No Hit
GTCATTCTTCACAGAAATAGAAAAATTACTGCTAAAATTTGTATGGAACC90.130001444460494No Hit
CAGGAGGGAGTTGAGCAGGACCGTCCCTGCGTGGTAGAGGACAGGGCAGA90.130001444460494No Hit
CTTGTCTTTGTGCCTTCATAGGGAGCATGGCCCCACTGACACCTTGATTT90.130001444460494No Hit
CTTATACTGATTTTCCATGAATGCGATTGTTTTAATAGTAACCTTTGCCA90.130001444460494No Hit
CTGAGAATGTCAGCATTAGAACCACCTGTAATGCCAAAGGACTTCCCTGA80.11555683952043912No Hit
GCCATGGCTAGGTTTATAGATAGTTGGGTGGTTGGTGTAAATGAGTGAGG80.11555683952043912No Hit
CCCTGCACTCGCGTTGATTCCATTCCATTCCATTCCATTCCATTCCGTTC80.11555683952043912No Hit
CCAGTAACTTAGTTCTGATCACTTGATGTTACAACAGGGATTTCTGGAAA80.11555683952043912No Hit
CCTGACAGGTGATCTGATAACTAAAATGTCAAGTAAGTGACTAACTGGCA80.11555683952043912No Hit
GAGGGCTTCATAACAGACGACAATGCACCGACGTAACTTTGACAAATCAC80.11555683952043912No Hit
GAGCGGAATCTCCATGTGCCAAGTCTAGTTCAAGAGACTGGATAGAGATT80.11555683952043912No Hit
GTAGAGATGGGGTTTGGGCATGTTTCCCAGGTTGATCTTGAACTCCTGGG80.11555683952043912No Hit
CTCCATACTTTAGTTTATGAGTTTCTTGGCAGAAAAACCTAATTTGCATA80.11555683952043912No Hit
GCACCAGAAAGACCAAGCCATGATTAGAAAATTGGAACTTTCCACCTCAC80.11555683952043912No Hit
CTCTGCCTCCCAGGTTCAAGCGATTCTCGTACCTCAGCCTCCCGAGTAGC80.11555683952043912No Hit
CTGTAATCTTTCACAGCACAGTAACAAAGTTATTAGGAAAACAGGACTAC80.11555683952043912No Hit
GTTTGGTTTTCTGGAGGTTTTGAAATGCATTCCTAGATCCTAACAGGCAA80.11555683952043912No Hit
CTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTT80.11555683952043912No Hit
GGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGG80.11555683952043912No Hit
TGAATATGAAAAGTGTGGACTCTAGCCCATACTGCCAGTGCCTGATTCAT80.11555683952043912No Hit
GGCCCACTTGGAGGAGGGGTCATGGTACTTCCAGGAAGTGAAGTAAAACC80.11555683952043912No Hit
GCACTTGGCTCCAGCATGTTGTCACCATTCCAACCAGAAATTGGCACAAA80.11555683952043912No Hit
GCCAGTATCAAAGAAGGGTCCATGTGATAAAAGAAGTGAAAAGCAGTCTT80.11555683952043912No Hit
CAAGGCTTTTCTCATCAAGGGGCGTATAGGCCAACATTGCTCCAATTCTT80.11555683952043912No Hit
GCAAAGGCACTGAGCATAAAAATACATAAAGTGCATTACCAATGAATAGT80.11555683952043912No Hit
GGTTTTTTTGATTTGTTGTAGTTGATTGACTGTGTCCTTTACAAAGACTT80.11555683952043912No Hit
CTTGTTGCAGATGAGAGAACTAAGGCTCCAGAGACTAAGCCAGGAAGCGC80.11555683952043912No Hit
GCTGCGGAGAAAGCTCTATGCTGAGAACATTCCCCTTGATGGAAAGAAGG80.11555683952043912No Hit
CTACATTTTGTTAATTACCATCAGTTGATGAATATTTTGACTAGAATTCA80.11555683952043912No Hit
GCCACTAAAATTACACGTTAAAAAAGAAAGTACATCACCCTTCCCGTGGT80.11555683952043912No Hit
GTAGATTTGGTTGTTCAGTAATATAAAATGTGTTTAAGAAAAAAAACAGC80.11555683952043912No Hit
CTTTTGACAAACAGGTTGTTTCCATATCTTGACTACTGTGAATAATGCTG80.11555683952043912No Hit
GGCGGTACTTAGTGTTCAACAGAAAACACTCCATCTGCTAAACAATATCT70.10111223458038424No Hit
GTCTGAGTGAGTTCTTCAGGGAAGCGATACTCTGAGTACCACAGGGACCA70.10111223458038424No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA70.10111223458038424No Hit
CCGATGATGAAGCTGAAGCAGAAGCAGATCCAGCCCAGGATGTAGGAATA70.10111223458038424No Hit
GTGCTGCTGCAGGACTGTTGACTTTTTGGGGGACACTGAGTCCTCCAGCA70.10111223458038424No Hit
ATTTTCTTTCTTGGGTGACAAAAAATGTTCTAGACTTATTGGTGATAGTT70.10111223458038424No Hit
CTTGTTTCTATCTTCCAATTTTCTTCTAAGTTTGTACATTTTGCCCTTAG70.10111223458038424No Hit
GTCATTATCAAGAATTATGTACCGTACATAACTGTATGTGCTGGACTTTG70.10111223458038424No Hit
GCCCGTAAGCCCGGCAGGCCTCTTTCAGCATGGGCACAGTGAACTTGCCC70.10111223458038424No Hit
GTCATAAATAATGGAAAACTCCAAACACCAGTCATGAAAAGTAAACTTAA70.10111223458038424No Hit
CAGGAGAATTCTGCAGTTGGTTCATTGTGCCTCCTGCTGTGTTGTGAAAT70.10111223458038424No Hit
CTTACATTTACCATTATAGAGAGGTCTTGTGGTTAGAAATTTAAAAAGTG70.10111223458038424No Hit
GTCACAAACTATCTTGCTTCTAGTCTCCAAGACTTTACTAAGTTTATCAA70.10111223458038424No Hit
CAATACTTGAAGCTTTCGCATACAGAAAATAAAACAAAAAGAAAAACAAA70.10111223458038424No Hit
GGCAAAAAAACACATGAAAAAATGCTCATCATCACTGGTCATCAGAGAAA70.10111223458038424No Hit
CCTGTCCTTATGTATAGTGTCCAACCTCTGATTCTAGCAGTCAAGTGTCT70.10111223458038424No Hit
GTCTTGGTGAAGAACCATTCCTCGGCATCCTTGCGGTTCTTCTCTGCCAT70.10111223458038424No Hit
GCCAAACCTATTGAGAAGCCTGCGGGCCAGCAGCAGGCCAGTACAATATG70.10111223458038424No Hit
GGCATTTTCATCTTGGGCATTTTCAGGTGCCAATCTGGGTCGTGAACCTC70.10111223458038424No Hit
CTTGAAGTCTCACCACGTTCTGGTGTTTTGCCCCATCCGGTCACCCAGCA70.10111223458038424No Hit
CTCGCATAATCTTTGAGATCTCTGCTCTGTGGATGTGCAGTTTTGATTTT70.10111223458038424No Hit
CTCCACACGTGGCGATTACAATTGGAGATAAGATCTGGCTGAGGACACAG70.10111223458038424No Hit
GGAGACAGCGCCGCCATCGTTACGCCATTCGTGCAGGTCGGAACTTACCC70.10111223458038424No Hit
CCTGTCTTTACAATCTAGTGCTGTTACTTTTCTTCTTGTGTGTACTCTGC70.10111223458038424No Hit
GCAAGTTTCTGTAAATTCCTCTGGGGTAGAGGGCAGACATCCAAGCAGTG70.10111223458038424No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT70.10111223458038424No Hit
GTCAGACTGAGGAGGGGGTACTACTGGTATCTAGTGAGTGGATACCAGGG70.10111223458038424No Hit
GGGGATTCGAATTTAATGGTTTGCAGAGTGGATGCCATGCTTCACAGTAC70.10111223458038424No Hit
GTAAATTTCTTCAAGGCCAAGTTTTATCATTGTTGCTAATATCCTTAGAG70.10111223458038424No Hit
CATTAGACTCATTCCAAATAATGATTTACCTGTGTTTGGTGCTCCATATA70.10111223458038424No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATATCGTAT70.10111223458038424TruSeq Adapter, Index 3 (97% over 36bp)
TCCTTTAGCTCCTTTACACTGTTTATATCTGCCTAGAAACTTCCAGCCCA70.10111223458038424No Hit
AGGCGAGGCTGCTCAGAGCCATCCCACTCCCAGAAACGAATGACACAAGC70.10111223458038424No Hit
TCCTCAGGTGTTTGCTGTCTTTCTTCAGGTTCCTCTACTTCTTCTTCAGA70.10111223458038424No Hit
TCTTTCTCTTGCTTGCTCTTTTTCTCAGACTTCTTCATTTGCTTTTCACT70.10111223458038424No Hit
CTAAGAGATTTTCGCGTACAGGGCTATCACCCACTATGGCCGCACTTTCC70.10111223458038424No Hit
GTGCAGTGACAAGCAAAATTTTTGCTCTCCAATTTCTGAAAGTTATATGA70.10111223458038424No Hit
GTCTGTTTTTAGTTAGGATCAGTTTTAAACAGGCACGGGCTTCTTCGAGA70.10111223458038424No Hit
CCCTGGGTTCCGCACATTAATATGCTCTCCTCGCTCTCTCTCTGTTTCCT70.10111223458038424No Hit
GTCCCATTTATTTTTGGCTCTTGGGGCGATGTCATCTTTTCAATATGAAA70.10111223458038424No Hit
CTCGGAAAGGTAAAATGTGATTTGAACATTTTCTTTTGTCCCTATTAAAT70.10111223458038424No Hit
CATCCTTTTAAAGTGCACACGCCACTGATTTTTGGTGTAGTCACAGAGTT70.10111223458038424No Hit
AGCTTCCAATAATTTTTTAATGACTATATCATCTAGTAGACATTAAGAAT70.10111223458038424No Hit
CCTTCATCTGTAGATGGACAGGAGATAAAGTTTTTGGAATCTCCATGCCT70.10111223458038424No Hit
CTTGTTTGTTTGTTTTGAGCCAGGGTCTCACTCTGTTCTTATGCTGGAAT70.10111223458038424No Hit
CGATCATCAATGGCTGACGGCAGTTGCAGCGAAGCAACGCCAAAAAGCCG70.10111223458038424No Hit
TGGAATAAATTTTTCACTTGTCAAGTATTTTTTTTTACTGTGCCTAATTG70.10111223458038424No Hit
CGGGTAGAATCCATCAGCGCCAGGCGTTGGCAGGGAAGCCAGCCCTGCAT70.10111223458038424No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)