FastQCFastQC Report
Tue 7 May 2024
EGAF00008325328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008325328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6815
Total Bases511.1 kbp
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATCTCGTAT3735.473220836390316TruSeq Adapter, Index 3 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG350.5135730007336757No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC310.4548789435069699No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCAGCTCGTAT150.22010271460014674TruSeq Adapter, Index 3 (97% over 36bp)
GAATTTTTGCCATTAGTTACTATACAAATGCTGCCTAGTGCCATTATCCA130.19075568598679382No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA120.1760821716801174No Hit
CATCAAATGAAGTGAATGACATTAGACTGTGGCAGAGTAAACCACATCTG110.16140865737344093No Hit
GTCTAAGGGTAAGGTATATACATGAAAGGGAAAGAAAGAGAATGAATTGT110.16140865737344093No Hit
CCTGTGAGTTTCTCATTAGTGATTATTATATTGCAGGCAGCCAAAATGGA100.1467351430667645No Hit
CTATCTTCTCTATTCTTTTCTTCGCCTTCCCGTACTTCTGTCTTCCAGTT100.1467351430667645No Hit
GCCACTCTTAACTCTTGAAGTAAATAAATAATCTTTGCTGGCAGGACTAT100.1467351430667645No Hit
CTCAGATCTCATAATCTTACCCTTCACTGAGAGGGACTTAACAATCTCGA100.1467351430667645No Hit
GTGCCATGAGTGCAAAATTACTATCCCACAATAGAAAAGACAAGGGGATC100.1467351430667645No Hit
CTCTCCTGTCCAGCTTGCCTCAGATCTTCAGGTTCTCTCTTCTCTGAGGC90.13206162876008803No Hit
CTTGCATTTTCAACTGTTATTGCTTTGGCCAAGAAGATTGCAGCATCCAT90.13206162876008803No Hit
GTCACAAACTATCTTGCTTCTAGTCTCCAAGACTTTACTAAGTTTATCAA90.13206162876008803No Hit
CTGCTGGCTTCTTCCTATACACTGTTCACCAATTTGAGTAAAGTGGACTT90.13206162876008803No Hit
GTAAATTTCTTCAAGGCCAAGTTTTATCATTGTTGCTAATATCCTTAGAG90.13206162876008803No Hit
CTTCTCCATTCATCCTCTTCTGAAGAACCAGAACCTTCACTCTGATCACA90.13206162876008803No Hit
CCTACACTCAAAGCAGTTTTTAGACCTCTTGTAGAAACTAATTTCCATGG90.13206162876008803No Hit
CTCCTGATGTCCAGGAAAGGAAAGAGAGCATGCCAAGACAGAAGGGAAAG90.13206162876008803No Hit
CCCACAGACAGGGTCATATATCTTGGTGCATCCATTAAGTTCATTGTAAC90.13206162876008803No Hit
GCCACTTGCTTTTCTGGCTAGGTTGCTAGGGTGCAACTCTAGGAAGTTAC90.13206162876008803No Hit
ATCAGTCTTCCCCACCTTGTAGCAAAGCAAAACAATTTTTGTTTGTTAGT80.11738811445341159No Hit
GTCTCGCTTGCCAGGACTTCTTGCTTTTGCACACAGCTTTTAAGAAGGGC80.11738811445341159No Hit
CTCTATATCATTTGTTTCCTAAACATCACGGGTATCTATCTAAACACAGC80.11738811445341159No Hit
TGAGAAATAGAAATCTAGAAAAGTGATCAAGGTTGGAGTTTAATGAGAAG80.11738811445341159No Hit
GTCTGAGGTATAAAAAAAAGCTGGAATATTGGCCTGCCTCCGGTAGGTAG80.11738811445341159No Hit
GTCGGTTCGGTCCTCCAGTTAGTGTTACCCAACCTTCAACCTGCCCATGG80.11738811445341159No Hit
ATGGTGAGCTCAGGTGATTGATACTCCTGATGCGAGTAATACGGATGTGT80.11738811445341159No Hit
TCCTTCTTTACCAATAAGACGTCCAACAAAGTTATTATGAGCTAAAATCT80.11738811445341159No Hit
GGAGGCCTGGATGGCTCCCTTCTACACGGGGACTTTGAATTCCACTGCAG80.11738811445341159No Hit
AAGCGGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACAGAA80.11738811445341159No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAATGCGCATATCGTAT80.11738811445341159TruSeq Adapter, Index 3 (97% over 36bp)
ATCCTTTTAAGTCAAAGCCATTGCAATTCTTCAATTCTAAATGGGAGGAG80.11738811445341159No Hit
CTCTTTTATACAAGAAAAAAAGGCAACAGTTGTTTTCTTTTTTTTCCCAA80.11738811445341159No Hit
CAAAAGGGGTGGTAGGATAATTTTTTTACAAATTTGCTTTAAATTCTACT80.11738811445341159No Hit
GGACCCTCATCCCCATTTCCAGTCTGATTATACAAGTGCTAAGTGGCAGA80.11738811445341159No Hit
GACCCCTTTCCTTATTCGGCTGCCACCACTGCTGCCCAAAATGCAGTAAT80.11738811445341159No Hit
CACCAGAGCTGGGGAGCTATTCCTCACATCTGAAACATCCTATTTCTTTA80.11738811445341159No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT80.11738811445341159No Hit
GCATTTATTTCAAAAGCCTTTGTTAAAGACTTACTTTCCCCACTGAATTA80.11738811445341159No Hit
CTCTAGGAGCTTCTGAACTATGAGTTCAAATGAAGAAGATAAGCAGTAAG80.11738811445341159No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC80.11738811445341159No Hit
GTTGTTTAGGGATATTTTTTTGAGACGGAGTTTCACTCTTGTTGCTCAGG80.11738811445341159No Hit
ATTTTGTTTATTAAAATTAAACCACAATCTGTCTCCTGACATACAAATAA80.11738811445341159No Hit
CTGTATTTGATTTTATTTGGTATATGCCACTATGACCTTATTTTAGTAGA70.10271460014673514No Hit
AGAAGTGATAATTATTCCCATCTGAAAAGAAATGCCACTTTCTCTGCAGG70.10271460014673514No Hit
GTCATAAATAATGGAAAACTCCAAACACCAGTCATGAAAAGTAAACTTAA70.10271460014673514No Hit
CCGTAGCCCCCCGATGACTCCATAGTGTAGCGCCTATTGCATTGCACACA70.10271460014673514No Hit
GTCACAAATAAACAAGCAAACAAACAAACACTGAGGTTGAGGATAATGCC70.10271460014673514No Hit
CCCTGTTCTCTCCTTCCTTGCCCTCTTCTTGGAATTGGAAGTATTTTTCA70.10271460014673514No Hit
TTCATCCAAAGCATGAATCAGGGAGGAAAGTGCAACTGCAGCTGCCTCAT70.10271460014673514No Hit
GGCAGCACTTTTATTTTTCCTTACACAATGACGTGTTGCTGGGGCCTAAT70.10271460014673514No Hit
GGCATTTTCATCTTGGGCATTTTCAGGTGCCAATCTGGGTCGTGAACCTC70.10271460014673514No Hit
GTAAGTTTATTCAATGCAAAAGAATCCTCTCCAATTTTACTGAGGTGGCT70.10271460014673514No Hit
CTCCAAATATATCATCACTAAGAAATCTTCATAGTTTAGATGAAGGAATG70.10271460014673514No Hit
TGTGGAAAAAAGAAGAAAAAAATTTATTGGGTGAATGAAAAGTGATTTTG70.10271460014673514No Hit
CCCTTATTCTCAAATCTCCACCACCTAATATTTCAGTAACGCCTCAATTG70.10271460014673514No Hit
AGAATGATATTTCCTAGTCACCTAAATTGCTTGTGCATGATAGGCATTAT70.10271460014673514No Hit
CTCCACACGTGGCGATTACAATTGGAGATAAGATCTGGCTGAGGACACAG70.10271460014673514No Hit
GTTCCAATGCCTGCTCCTCAGCATACACGAGCGTACGTAGACGTGCGCAC70.10271460014673514No Hit
GGGGACTTAAAAAAAAATTAAAGACAGTACATTTATTTAAACTGTCCCTT70.10271460014673514No Hit
CACGATACCAATTTTATCTGAGGTGACTACATCTTCATTTACATCTATTG70.10271460014673514No Hit
GGGGTTGGGGGCCTCTCGGCTGTAGTACTCATGGATCAGCACAGAGTGCT70.10271460014673514No Hit
GTGCTTGATGACAGAGACAGATTCACCTGTGAGAATTGACTGGTCAACTC70.10271460014673514No Hit
GGCCAACATGGTGACACCCAGTCTCCACTAAAAATATAAAAACTAGCCAA70.10271460014673514No Hit
GTCCATACTGATACACGTTGTTAGCCGCAGAATTCAGGGCTCTTTGGACT70.10271460014673514No Hit
ATTCCACTAATACTCGCCTATGATGCCAAACTTGATAGTTTTTGGGCTGC70.10271460014673514No Hit
CTTAGCCTCCCGAGTAGCAGGGACCACAGGCGTGCGCTGCTATGACTGGC70.10271460014673514No Hit
GTGGCTTATCAACTAGGGGTCCTTCATACTAAAGCAAACCAAGTCTTTAC70.10271460014673514No Hit
GCTCATTTCGGGAAGCTTCTATCTTTTCACGATCTGCAGCATCTATCATG70.10271460014673514No Hit
CTAAGAGATTTTCGCGTACAGGGCTATCACCCACTATGGCCGCACTTTCC70.10271460014673514No Hit
CCATAAACCAACAAATAATTTGGCTGTAATGTATCATAAAACACAAACCC70.10271460014673514No Hit
CCCTGGTCATCCTGGGCTTCAAGGTTAGATACATAAGAGTAAGAAATCAG70.10271460014673514No Hit
GCCTAAGTTGTATCCAGCTTCCCTGTGTATTTGTACTGCTTTCTCCCATT70.10271460014673514No Hit
CAGGAGGGAGTTGAGCAGGACCGTCCCTGCGTGGTAGAGGACAGGGCAGA70.10271460014673514No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC70.10271460014673514No Hit
CTCAGGGCCAGTTTATAAATACTCCATTCAGTACTATCTTAACACAAATC70.10271460014673514No Hit
CTCCTGATGACTTTCCAGCCTTTGACCTCAGCTCTATTTCAAATGCCTTC70.10271460014673514No Hit
CCTTGGAATGTTCATTTCTTTGGCTTACAGGAGAGACTAGACAGGAAGGC70.10271460014673514No Hit
CTTGATTTTCAAAAAGTCAATGCCATCCCATTAAACAAGTAACCCCTTAG70.10271460014673514No Hit
GTCCACAATAATAATACACAATATTATGAGCCTAATACAACAAAGTTGTC70.10271460014673514No Hit
CTTACATCTTCCTCGGATGGCATCAGTAGCCTCCCTTACAGACACTGCAC70.10271460014673514No Hit
GGCATGGATATCTTTGGAGTCTTAAAATGCATCTCTGGCATCTTAAACTT70.10271460014673514No Hit
GGCATAAGTTTCATAAGTACTCTTGCTCCATCTCTAAGGGGTGGCATATT70.10271460014673514No Hit
GTGTCCTTTAGCACTTAAAGCCAGCTCTATCTATAAAGCAAGAAAACAGT70.10271460014673514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)