FastQCFastQC Report
Sat 11 May 2024
EGAF00008326905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008326905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22567726
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4554742.018253855084912No Hit
CACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCA1994800.88391714787746No Hit
GTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGG1897490.8407980493914184No Hit
CTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC1896420.8403239209834432No Hit
AGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATC1311560.5811662194055351No Hit
CCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTC1240500.5496787757880435No Hit
GGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCA1184330.5247892499226551No Hit
CAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAG1134880.5028774277036153No Hit
GGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGA1083730.4802123173597552No Hit
GGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAAC1078200.4777619154007808No Hit
ACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAG1039810.4607508970996901No Hit
GATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG901660.39953515919149324No Hit
CTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCC788250.34928197905274105No Hit
CCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTT784710.34771336731046804No Hit
GGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTT770740.3415231113670912No Hit
AGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGT758660.33617033457425No Hit
CTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT736830.326497228830233No Hit
GATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGG730910.3238740137132115No Hit
GGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCAT703910.3119100258484173No Hit
CATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACC684450.3032870923725324No Hit
ATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTA660800.292807525224296No Hit
GGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACG641580.28429093830720914No Hit
GGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCT641440.28422890281457686No Hit
GTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCG624170.2765763816877252No Hit
GGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCC622590.27587626684230393No Hit
CTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCT615960.2729384431555044No Hit
CAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGT611040.27075833870014193No Hit
CTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCG586520.2598932652762622No Hit
ACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACT566960.2512260207342113No Hit
GGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGT566960.2512260207342113No Hit
GGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT560990.24858065008410685No Hit
GTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCC559380.24786724191883575No Hit
GATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCT551620.2444287031843616No Hit
ATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT550120.24376403719187303No Hit
CCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACC531170.23536709015343416No Hit
GGACGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACG526020.23308507024589006No Hit
GGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTG521220.23095813906992665No Hit
ATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAAT519980.23040868184946947No Hit
GTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCGCCTGTGAATAGCCA518610.22980162024299655No Hit
GGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAA518040.2295490471658509No Hit
CTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAA486370.215515732511109No Hit
GGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGA479870.21263551321032523No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA478550.2120506071369353No Hit
CATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCAGGTTGCCTAAGG458370.20310863398465576No Hit
CGGGGGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAA456180.2021382216356225No Hit
CTAAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTC452080.2003214679228204No Hit
ATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGT442970.1962847297951065No Hit
CCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCG438070.19411348755297722No Hit
GTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTG437970.1940691764868113No Hit
GTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGAT431020.19098955738828097No Hit
ACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGT429880.19048441123398965No Hit
CTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCT418200.185308878705812No Hit
CATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCC401640.17797096614873825No Hit
GGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCA393540.17438176978929998No Hit
TGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGG393050.17416464556508707No Hit
CCCAGCTACTCGGGAGGCTGAGGTGGGAGGATCGCTTGAGCCCAGGAGTT390930.17322525096236988No Hit
GTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGAC383470.16991964542639343No Hit
GTTCTTATCAGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATAT380410.16856372680171675No Hit
CGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGT376510.16683559522124647No Hit
CTACAATATAGCTGCTAAGTGCTGTGTTGTCGTTCCCCCTGCTTAAAATA375880.1665564355044013No Hit
CACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGGGACCACCA371380.1645624375269356No Hit
GTGGGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCC368660.16335717652722298No Hit
CGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTAGTGGGATCGCG365170.16181072031803292No Hit
GTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAG364380.1614606628953223No Hit
GATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGC359300.15920966073409434No Hit
GAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCA357670.1584873903555901No Hit
CGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCTGGGCTGTA349310.1547829852241205No Hit
CGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGT339770.15055570951189323No Hit
GGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCT337740.14965619486872536No Hit
ATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCG331680.14697094425907156No Hit
AAGATCAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCT329490.14600053191003826No Hit
CTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTC320570.1420479848080396No Hit
GGAGGATCGCTTGAGCCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGAT318570.14116176348472148No Hit
ATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCG313960.13911902333447332No Hit
CAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCCAGGAGTTCT313740.13902153898890832No Hit
AACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAG311980.13824166422438838No Hit
GTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAA306270.13571150234631527No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN295890.1311120136782944No Hit
CCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGG295400.13089488945408145No Hit
AGGACGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATAC290310.12863945618623693No Hit
GGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTC289780.12840460753555766No Hit
ATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTG283220.12549780159507432No Hit
GGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGCGGGG281030.12452738924604101No Hit
CTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCACCGAAAA280780.12441661158062625No Hit
CGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAAGGTCC280290.12419948735641331No Hit
GGTGGCGCGTGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGA279210.12372092784182155No Hit
GGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAG274520.12164273883864063No Hit
GGACCACCAGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGG273140.12103124612555115No Hit
AGGTTGCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTC271180.12016274922869942No Hit
CAGGGGAGATACCATGATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTAT265920.11783198714837287No Hit
ATCGGGTGTCCGCACTAAGTTCGGCATCAATATGGTGACCTCCCGGGAGC262420.1162810998325662No Hit
CAAGTGTAGTATCTGTTCTTATCAGTTTAATATCTGATACGTCCTCTATC254170.11262543687387909No Hit
GTCGAAGAGGACGACCATCCCCGATAGAGGAGGACCGGTCTTCGGTCAAG254100.11259441912756298No Hit
CAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTA253810.11246591703568183No Hit
GTTTCTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCG249980.11076880320152771No Hit
GTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAGCTCTCAA243420.10786199726104438No Hit
GACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAA242520.10746319766555125No Hit
GATCACGAAGGTGGTTTTCCCAGGGCGAGGCTTATCCATTGCACTCCGGA241740.10711757134945718No Hit
ATCATTTCTATAGTGTGTTACTAGAGAAGTTTCTCTGAACGTGTAGAGCA240110.10639530097095296No Hit
GTCAAGGGTATACGAGTAGCTGCGCTCCCCTGCTAGAACCTCCAAACAAG239390.10607626129455842No Hit
CGATAGAGGAGGACCGGTCTTCGGTCAAGGGTATACGAGTAGCTGCGCTC236410.10475579152281447No Hit
GGATCGCTTGAGTCCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGG235250.10424178315529001No Hit
CTGTAGTCCCAGCTACTCGGGAGGCTGAGGCTGGAGGATCGCTTGAGTCC234990.10412657438325865No Hit
CCAGGAGTTCTGGGCTGTAGTGCGCTATGCCGATCGGGTGTCCGCACTAA233190.10332897519227237No Hit
CCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACT230190.10199964320729522No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)