FastQCFastQC Report
Wed 8 May 2024
EGAF00008326963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008326963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16086714
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG2523711.5688163536692452No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2463581.5314376820524067No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2369711.4730851807274004No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT2014931.252542936985142No Hit
CCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTG1744101.0841866151160517No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1652341.027145755186547No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1603610.9968536768913776No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGA1396640.8681947102434966No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1287250.8001944959051303No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1185810.7371362479621382No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1030200.6404042491213556No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1025750.6376379912019323No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACGA1009640.6276235159026262No Hit
CAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAG967170.6012228476244434No Hit
GCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT915450.5690720926598185No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT876410.5448036186880677No Hit
CCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC829600.5157050718997055No Hit
GTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT786250.4887573683475693No Hit
CTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGC785660.48839060606162327No Hit
CTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGT775050.481795101224526No Hit
GTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACT629790.3914969831626272No Hit
GGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCA597080.3711634333773821No Hit
GGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACC592060.3680428457918752No Hit
CACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCAC577280.3588551397134306No Hit
CTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC528070.3282646785415592No Hit
CTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGT501000.31143712755756087No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA496480.30862735546861836No Hit
GGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAA490040.3046240518728685No Hit
CACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT475230.29541769686463No Hit
GTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA440030.27353628590649404No Hit
GGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAG426440.26508832070987276No Hit
TGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGAC406210.25251272571887584No Hit
CCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCC398130.2474899472943946No Hit
ACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTG393400.2445496326968951No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC393120.24437557601881899No Hit
ACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACT388730.2416466159589833No Hit
GCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCT388500.24150364083056364No Hit
GGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCG386640.240347407183344No Hit
CCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAGTGCGGACACC380460.2365057276458076No Hit
CCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTC369540.22971751720084038No Hit
CGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCC358000.2225438955401333No Hit
GTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGA348320.21652650752664587No Hit
GGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCG342500.2129086151466359No Hit
CGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCT342180.2127096932288347No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAACG334930.20820286852865044No Hit
GTCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGAT328620.20428037696200727No Hit
CTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACG310850.19323399421410736No Hit
GGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTT296770.18448142983085294No Hit
CCCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCG296190.1841208838548382No Hit
CACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGGGAGAAGAAC292050.18154733154328473No Hit
CCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGG267690.1664044005506656No Hit
CCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAACTTAG266170.16545952144110973No Hit
CTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTC265890.16528546476303363No Hit
TGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTAC250300.15559423758015467No Hit
GTCTGACCTCGCGCGGAGCCCCGTTCTCTGGGAACTCACCTCCCCGAAGC249170.15489179455791904No Hit
CCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCT227300.1412967247381908No Hit
GGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATGCCGAA227290.1412905084282595No Hit
CCCGTTCTCTGGGAACTCACCTCCCCGAAGCTCAGGGAGAGCCCTGTTAG223770.1391023673324459No Hit
GACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCT221360.13760423663900534No Hit
CTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCGCAGCTACTCGTATA214160.13312849348847752No Hit
CCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGG212690.13221469592857807No Hit
GCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTACAGCCCAGAA209950.130511427007405No Hit
CACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGC209270.13008871793207735No Hit
GTCACCATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGC208880.1298462818447571No Hit
GCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCAC205970.12803733565475212No Hit
CGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGC205110.12750273300066128No Hit
GGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG204010.12681893890821955No Hit
GGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTA202820.1260791980263962No Hit
GACGGGGTCTCGCTATGTTGCCCAGGCTGGAGTGCAGTGGCTATTCACAG199980.12431376600591022No Hit
CTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTG198230.12322591176793471No Hit
CACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGCAGGGGTCA181710.11295656776144589No Hit
CTCCTCTATCGGGGATGGTCGTCCTCTTCGACCGAGCGCGCAGCTTCGGG181560.11286332311247653No Hit
CATATTGATGCCGAACTTAGTGCGGACACCCGATCGGCATAGCGCACTAC181130.11259602178543113No Hit
CGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGC180020.11190601138305808No Hit
GGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCG179650.11167600791560041No Hit
CCCACTACCACAAATTATGCAGTCGAGTTTCCCACATTTGGGGAAATCGC177690.11045761116906783No Hit
GTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCC176100.10946921788999296No Hit
GCCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATA173210.10767270431985053No Hit
GACGGGGTCTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAG170830.10619322255620382No Hit
GCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGG163600.10169883047588214No Hit
ACTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACG162150.10079746553584529No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)