FastQCFastQC Report
Tue 7 May 2024
EGAF00008337769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008337769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27461
Total Bases4.1 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGCAATTGATCT6502.3669931903426678No Hit
ATTGCGCAATGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.9759295000182076No Hit
ATTGCGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGCAATT2040.7428717089690834No Hit
ATCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGCAATTGATCG1980.721022541058228No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTG1630.5935690615782382No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1590.579002949637668No Hit
ATTGCGCAATGACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAG1440.5243800298605295No Hit
ATTGCGCAATGTATGCTGCTGATGCTCGTGGGAAAAAAAAAAAAAAAAAA1410.5134554459051018No Hit
ATTGCGCAATGTATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGAC1350.4916062779942464No Hit
ATTGCGCAATGATGCTCGTGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA1200.4369833582171079No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGA1190.4333418302319653No Hit
ATTGCGCAATGTATGCTGCCTGTCTCTTATACACATCTCCGAGCCCACGA1150.4187757182913951No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCT1140.41513419030625254No Hit
ATTGCGCAATGCGCTACGGGGGGGACCGACTTCATCACTAAATCTATACT980.3568697425439714No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCT870.31681293470740324No Hit
ATTGCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGCAATTGAT870.31681293470740324No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTC850.3095298787371181No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGAT800.2913222388114053No Hit
ATTGCGCAATGCCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAG790.28768071082626273No Hit
ATTGCGCAATGCTCTCGTGGGGCAAAAAATAAAGTATTTAATCTTGGTAC780.2840391828411201No Hit
ATTGCGCAATGGCTACAATGGGGTGGTCATGGAGTTCCCCTCTCTGACTA720.2621900149302647No Hit
ATTGCGCAATGGTTACCTTGGGCCAGATTGCCATTGCAAAATTAAAAGAT710.25854848694512217No Hit
ATTGCGCAATGCGTAACGGGGGGGATAGGAGCGGCCGGACTTCAATTTAC700.2549069589599796No Hit
ATTGCGCAATGCTTACCGAGGGGGACCTCTTCATTTACTACCACTAGACA690.25126543097483706No Hit
ATTGCGCAATGTTCGAAGTGGGTACCTGGTTGATCCTGCCAGTAGTCATA670.2439823750045519No Hit
ATTGCGCAATGATGCTCGCTGTCTCTTATACACATCTCCGAGCCCACGAG660.24034084701940933No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGTCTC640.23305779104912422No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTG570.20756709515312627No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGC570.20756709515312627No Hit
ATTGCGCAATGGATGTACGGGGGGTATCGAAAACCCACTTGTTCGCTCGG540.19664251119769854No Hit
ATTGCGCAAAAACTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAG520.1893594552274134No Hit
ATTGCGCAATGTATGCTGCTGATGCTCGTGGGGAAAAAAAAAAAAAAAAA520.1893594552274134No Hit
ATTGCGCAATGCAGTAACCGGGAGGCATCATGGCGTTCACAGCGGGACCC500.1820763992571283No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCT480.17479334328684315No Hit
ATTGCGCAATGTCTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAG470.1711518153017006No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCCTG470.1711518153017006No Hit
ATTGCGCAATGCTGCTGATGCTCGTGGGAAAAAAAAAAAAAAAAAAAAAA460.16751028731655804No Hit
ATTGCGCAATGAATCTAACGGGGAACTATAGAGTTTGATCCTGGCTCAGA460.16751028731655804No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCCTGT460.16751028731655804No Hit
ATTGCGCAATGCGGCGGTAGGGGAGGTGACCCTGTCATCACCAATGCCCA460.16751028731655804No Hit
CTGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGCAATTGATCTCG450.16386875933141545No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCTG440.1602272313462729No Hit
ATTGCGCAATGCGGATACCGGGGGCTCTTCTCATTATGGCACAATCAGCC420.15294417537598778No Hit
ATTGCGCAATGTGTCAAGCGGGGGTTTGCCGTCGTCCAAGATGGCTCCAA420.15294417537598778No Hit
ATTGCGCAATGTAACCAGGGGGATACCAAACGTATTATACTGGTATTTCT420.15294417537598778No Hit
ATTGCGCAATGCAGCCACCGGGCAAAGATCTACACAAAATCCATACCGCA390.14201959142056006No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCT390.14201959142056006No Hit
ATTGCGCAATGCATGGGCTGGGAGACATACTTGGACGATGTCGACTGCAA380.1383780634354175No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGT380.1383780634354175No Hit
ATTGCGCAATGGCAGGAGTGGGGAATTTTGGACAATGGGCGCAAGCCTGA360.13109500746513236No Hit
ATTGCGCAATGGGTTACGTGGGGGGGCGGCACATCTGTAAAACCATAACG360.13109500746513236No Hit
ATTGCGCAATGAAAAAAAAGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.13109500746513236No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGC360.13109500746513236No Hit
ATTGCGCAATGATGCTCCTGTCTCTTATACACATCTCCGAGCCCACGAGA350.1274534794799898No Hit
ATTGCGCAATGATGTGCAGGGGGAAGTGACAATCATCTGAATCACATCAG350.1274534794799898No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTC350.1274534794799898No Hit
ATTGCGCAATCTGTCTCTTATACACCTGTCTCTTATACACATCTCCGAGC350.1274534794799898No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGA340.12381195149484724No Hit
ATTGCGCAATGTGAGTCTGGGGAGCGGGTCTCGGGTTCCAAGTTCAGTTC340.12381195149484724No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCC340.12381195149484724No Hit
ATTGCGCAATGGTCAGAGTGGGGAGGTGAGACGGATTTGGCTGCCGCGTG330.12017042350970467No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCCTGT320.11652889552456211No Hit
ATTGCGCAATGTATGCTGCTGATGCTCTGTCTCTTATACACATCTCCGAG320.11652889552456211No Hit
ATTGCGCAATGCACAGTTCGGGGAGCTTCTTGCTTCTTGATACACCCATT310.11288736753941955No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAATCGTATGCTGCTGATG310.11288736753941955No Hit
ATTGCGCAATGATGCTCGTCTGTCTCTTATACACATCTCCGAGCCCACGA310.11288736753941955No Hit
ATTGCGCAATATCGCTCTGGGGCTCCATCTGGTCAGACTCCCAAGATCAA310.11288736753941955No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCCTGTCT310.11288736753941955No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATAGTATGCTGCT300.10924583955427697No Hit
ATTGCGCAATGCACTGGTCGGGCATCACACCCTATACGTCCACTTTCGTG300.10924583955427697No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGCTG300.10924583955427697No Hit
ATTGCGCAATGCAAGGAAAGGGGACAGACCAATGGAACACAATAGAGTCC290.10560431156913441No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAACGTATGCTGCTGAT280.10196278358399186No Hit
ATTGCGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGTATGCTGC280.10196278358399186No Hit
ATTGCGCAATGGGTGTGCTGGGTCTGGCCTCAAATCAGGTAGGGCTACCC280.10196278358399186No Hit
ATTGCGCAATGTCACCGGGGGGACTTCGTACAGCTTTTCCTCCCTTGCAC280.10196278358399186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)