FastQCFastQC Report
Tue 7 May 2024
EGAF00008339682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008339682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1101
Total Bases166.2 kbp
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGAGAATCACCGGGGCTTCTTCCCGGGAGGCCGGCCGGCCCCTGTCTCT30.2724795640326975No Hit
CCATATGAGGCCGACCACCGCCCAAGCTGATAATCTCAAACTCGTGCGCC20.18165304268846502No Hit
GCTCCCGTCGCGCCGCTGCCGAGCCAGGCGCCGCCGCAGTGGCTCCTGTC20.18165304268846502No Hit
ACGCCAGAGGAATTACGTCACCTTTGTGACGAAATGGTGAGCTTGGCTCA20.18165304268846502No Hit
TTTTTATTCTTGTTGAGTTTTTTCAGCGCCTCGATGTCCATGTGGGGGAT20.18165304268846502No Hit
GTCGATCGAGCTTGCGGCTGACACCCTTTGGGATCTTGGGCTTAACCTCC20.18165304268846502No Hit
ACACACACATGCGCACACACACACACAATTTTGAAACTGATAGAGAAAAG20.18165304268846502No Hit
GTGACGGAAATCTCTGTTGAGAATGTGGACACTAGAAACTCTGGGGATAT20.18165304268846502No Hit
CTGACAGTCTTTACATTTCCAGCTCTGTTCTTTCTCTGTCTCTTATACAC20.18165304268846502No Hit
CTCGTAGCTTTCGAAAAAATGGAGGCGGTCCGCAACAGTCAGTTTCTCAA20.18165304268846502No Hit
TTATAGTGCTTGTAACTTTATGTTATGCAAATATTCCCACTAACGTCTGT20.18165304268846502No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)