FastQCFastQC Report
Wed 8 May 2024
EGAF00008340411

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008340411
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences148412
Total Bases22.4 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGG7160.4824407729833167No Hit
GTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGG4590.30927418268064577No Hit
GAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGG4520.30455758294477536No Hit
GTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGGTGATGCCTGTTGGGG4520.30455758294477536No Hit
GTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAAT4140.27895318437862165No Hit
GTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTGTTGA3920.26412958520874324No Hit
CTATTAGACTATGGTGAGCTCAGGTGATTGATACTCCTGATGCGAGTAAT3540.23852518664258957No Hit
GTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGATAGAG3330.22437538743497829No Hit
GATTAGGACGGATCAGACGAAGAGGGGCGTTTGGTATTGGGTTATGGCAG3280.22100638762364228No Hit
GGTCTACGGAGGCTCCAGGGTGGGAGTAGTTCCCTGCTAAGGGAGGGTAG3140.21157318815190146No Hit
GGGTAAATACGGGCCCTATTTCAAAGATTTTTAGGGGAATTAATTCTAGG2860.19270678920841983No Hit
CCCTAAGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGT2780.18731638951028218No Hit
CCACTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAG2720.18327358973667895No Hit
GTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAGTAGGACT2620.17653559011400696No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCGTATAGGAGTGTA2540.17114519041586934No Hit
GGTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAG2520.16979759049133494No Hit
GACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTAAGTTA2420.1630595908686629No Hit
GTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGAAG2410.1623857909063957No Hit
AGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTATAGCAGATG2400.1617119909441285No Hit
TATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATATCATAG2360.1590167910950597No Hit
TCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTATTAGGACTT2350.1583429911327925No Hit
GGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATG2340.1576691911705253No Hit
CTCCTGATGCGAGTAATACGGATGTGTTTAGGAGTGGGACTTCTAGGGGA2270.1529525914346549No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATAT2220.14958359162331888No Hit
CTATAGCAGATGCGAGCAGGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAG2220.14958359162331888No Hit
AAGTAGGACTGCTGTGATTAGGACGGATCAGACGAAGAGGGGCGTTTGGT2200.14823599169878449No Hit
ACGTAGTACGTGTCGTGTAGTACGATGTCTAGTGATGAGTTTGCTAATAC2170.14621459181198287No Hit
GTATACCCCCGGTCGTGTAGCGGTGAAAGTGGTTTGGTTTAGACGTCCGG2150.14486699188744845No Hit
GAGTAGTTCCCTGCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCC2140.14419319192518126No Hit
AGATAGAGGAGACACCTGCTAGGTGTAAGGAGAAGATGGTTAGGTCTACG2130.14351939196291405No Hit
GTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCGTAGGTT2120.14284559200064684No Hit
GGGTAATAATGACTTGTTGGTTGATTGTAGATATTGGGCTGTTAATTGTC2080.14015039215157804No Hit
GTAAATATATGGTGTGCTCACACGATAAACCCTAGGAAGCCAATTGATAT2030.13678139234024203No Hit
GTAATAAGCAGTGCTTGAATTATTTGGTTTCGGTTGTTTTCTATTAGACT2010.13543379241570763No Hit
CATCAATAGATGGAGACATACAGAAATAGTCAAACCACATCTACAAAATG2000.13475999245344042No Hit
CCTAATTGGGGGGTAGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAA1920.1293695927553028No Hit
GTCCCAATGTATGGGATGGCGGATAGTAAGTTTGTAATTACTGTGGCCCC1920.1293695927553028No Hit
GTGTAAGGAGAAGATGGTTAGGTCTACGGAGGCTCCAGGGTGGGAGTAGT1910.1286957927930356No Hit
GCTAAGGGAGGGTAGACTGTTCAACCTGTTCCTGCTCCGGCCTCCACTAT1910.1286957927930356No Hit
GTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGA1840.12397919305716519No Hit
GTAAAATAGAGACCCAGTAAAATTGTAATAAGCAGTGCTTGAATTATTTG1830.12330539309489798No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT1830.12330539309489798No Hit
GAATATAAACTTCAGGGTGACCGAAAAATCAGAATAGGTGTTGGTATAGA1820.12263159313263079No Hit
GCTCATGAGTGCAAGACGTCTTGTGATGTAATTATTATACGAATGGGGGC1810.12195779317036358No Hit
GTCTACTGAGTAGCCTCCTCAGATTCATTGAACTAGGTCTGTCCCAATGT1780.11993639328356197No Hit
GCTCAGACCATACCTATGTATCCAAATGGTTCTTTTTTTCCGGAGTAGTA1780.11993639328356197No Hit
GTATAATACTAAGTTGAGATGATATCATTTACGGGGGAAGGCGCTTTGTG1710.11521979354769156No Hit
CTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGAGA1710.11521979354769156No Hit
TGCTAATACAATGCCAGTCAGGCCACCTACGGTGAAAAGAAAGATGAATC1670.11252459369862275No Hit
AGCTAGGACTGGGAGAGATAGGAGAAGTAGGACTGCTGTGATTAGGACGG1660.11185079373635554No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACATTAGTTC1650.11117699377408835No Hit
GAGTAATACGGATGTGTTTAGGAGTGGGACTTCTAGGGGATTTAGCGGGG1650.11117699377408835No Hit
GGGTAGAGGGGGTGCTATAGGGTAAATACGGGCCCTATTTCAAAGATTTT1620.10915559388728674No Hit
GGGTATAATTGTCTGGGTCGCCTAGGAGGTCTGGTGAGAATAGTGTTAAT1610.10848179392501955No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGATTCTATCT1600.10780799396275234No Hit
GGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAATGAAGCCTCCT1600.10780799396275234No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTT1580.10646039403821794No Hit
GCTGTGATTAGGACGGATCAGACGAAGAGGGGCGTTTGGTATTGGGTTAT1570.10578659407595073No Hit
GTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGGAGGAG1570.10578659407595073No Hit
GTATAGGGCTGTGACTAGTATGTTGAGTCCTGTAAGTAGGAGAGTGATAT1550.10443899415141633No Hit
GTGTAGTACGATGTCTAGTGATGAGTTTGCTAATACAATGCCAGTCAGGC1550.10443899415141633No Hit
GTTCAACCTGTTCCTGCTCCGGCCTCCACTATAGCAGATGCGAGCAGGAG1520.1024175942646147No Hit
GATCAGACGAAGAGGGGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTAT1520.1024175942646147No Hit
GAGCTGTTCCTCTTTGGACTAACAGTTAAATTTACAAGGGGATTTAGAGG1510.10174379430234752No Hit
GTAATAGGGCAAGGACGCCTCCTAGTTTGTTAGGGACGGATCGGAGAATT1500.10106999434008031No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)