FastQCFastQC Report
Wed 8 May 2024
EGAF00008342781

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008342781
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161583
Total Bases24.3 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32291.9983537872177148No Hit
GCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGC15170.9388363874912584No Hit
GCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12460.7711207243336242No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGTTCTAGAGTGTA10290.6368244184103526No Hit
CATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7600.4703465092243615No Hit
AGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATAC6340.3923680090108489No Hit
CATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGA6290.38927362408174127No Hit
CAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.33171806440033913No Hit
GCTAAGAGCATCGAGGGGGCGCCGAGAGGCAAGGGGCGGGGACGGGCGGT2180.13491518290909316No Hit
AGCATCAGCAGCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170.13429630592327163No Hit
CGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2080.12872641305087787No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG2070.12810753606505634No Hit
ATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAG2030.12563202812177024No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA1960.12129988922101953No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA1920.11882438127773343No Hit
GCGCAATCTGTCTCTTATACACATCTGACGCTGCCGACGACCGTTCTAGA1840.1138733653911612No Hit
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTTTTTTTTTTT1770.1095412264904105No Hit
GCAGCATACGAGCATCAGCAGCATACGAGCATCAGCAGCATACGAGCATC1770.1095412264904105No Hit
CCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCT1690.10459021060383827No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)