FastQCFastQC Report
Tue 7 May 2024
EGAF00008342918

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008342918
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1273
Total Bases192.2 kbp
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCATACGATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30.2356637863315004No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30.2356637863315004No Hit
GGATATGAAGGTCCTTTTCAAGGAGAACTACAAACCACTGCTCAAAAAAA30.2356637863315004No Hit
CGTAATGTGTACGCCGCAAATATTATCGAGCGCGTCGCCGTGCTGAGACC20.15710919088766695No Hit
CTTCTGTCTGTCGGAGCTCTCAGCGGAGCTCTCCTCCTCGGCGCCAGCGC20.15710919088766695No Hit
TCCCACTTCCTCGGCTGCATTTGGCAGGAACAAAGGGTTCATATCTGTCT20.15710919088766695No Hit
GGAGTAGGAGAGAGGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGG20.15710919088766695No Hit
GTTGCATTTTTCCAGGTGAGGCCTAGTAGGAAGGCAGCGAATGGGCCCGC20.15710919088766695No Hit
ATCTGTCTCTTATACACATCTGACGCTGCCGACGATCAGACTTCGGTGTA20.15710919088766695No Hit
AGGACTGCCTGAGCCCAGGAGGCGAAGGTTGCAGTGCAATGAGATCACAC20.15710919088766695No Hit
CCTATTGATAGGACATAGTGGAAGTGGGCTACAACGTAGTACGTGTCGTG20.15710919088766695No Hit
GATCTACACGTACGGTGAAACACTCTTTCCCTACACGACCTGTCTCTTAT20.15710919088766695Illumina Single End PCR Primer 1 (95% over 21bp)
GATAAATAGTTCGGCCACGACTTGTGGGTTTTGGCTGAGTTTGATGCGGG20.15710919088766695No Hit
GGTTCTCCCGGGACACCAGCAGATCCACACCCTGGGCACCTCCGTGTTTG20.15710919088766695No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)