FastQCFastQC Report
Wed 8 May 2024
EGAF00008389639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008389639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17957
Total Bases2.7 Mbp
Sequences flagged as poor quality0
Sequence length142-151
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA242413.498914072506544No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA223212.429693155872362No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA14478.058138887341984No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA7634.249039371832711No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGGGCGTA6893.836943810213287No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGGGCGCA5372.9904772512112268No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGTA3421.904549757754636No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA3391.887843180932227No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA3081.715208553767333No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA2431.3532327226151362No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGAGCGCA2061.147184941805424No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1540.8576042768836665No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGCA1540.8576042768836665No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAGGGGGCGCA1530.8520354179428634No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGGTTAAAGGGGGCGTA1380.7685025338308181No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGCGTA1340.7462270980676059No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1280.7128139444227878No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA1070.5958679066659242No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA1070.5958679066659242No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA1060.5902990477251211No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA1060.5902990477251211No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA960.5346104583170909No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGTGTA840.4677841510274545No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA840.4677841510274545No Hit
TACGTAGGTGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGTGTA800.4455087152642424No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA740.4120955616194241No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA700.38982012585621206No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGTA700.38982012585621206No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGGGGGCA670.37311354903380295No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGGGCGCA660.36754469009299995No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAAGGGGGGCA650.3619758311521969No Hit
TACAGAGGTCTCAAGCGTTGTTCGGAATCACTGGGCGTAAAGCGTGCGTA610.3397003953889848No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGCGCA590.3285626775073788No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGGGGGTA560.3118561006849696No Hit
TACGTAGGGGGCGAGCGTTGTCCGGAATGATTGGGCGTAAAGGGCGCGTA550.3062872417441666No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGGTTAAAAGGGGCGTA520.28958066492175755No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAGGGGGGGCA510.2840118059809545No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA490.27287408809934843No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA490.27287408809934843No Hit
TACGTAGGTGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGGGCGCA440.2450297933953333No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA430.23946093445453026No Hit
TACGTAGGGTGCGAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA430.23946093445453026No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAAGGGGCGCA390.21718549869131815No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAGGGAGCGCA380.21161663975051512No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGGTTAAAAGGGGGGTA380.21161663975051512No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA360.20047892186890906No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGGTTAAAAGGGGGGGA350.19491006292810603No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGGGTA340.189341203987303No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA330.18377234504649997No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA310.17263462716489392No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAAGGGGCGTA310.17263462716489392No Hit
TACGTAGGTCCCGAGCGTTGTCCGGATTTATTGGGCGTAAAGCGAGCGCA310.17263462716489392No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGGGCGTA300.1670657682240909No Hit
TACGTAGGTTGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA300.1670657682240909No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGGGCGTA290.16149690928328786No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA280.1559280503424848No Hit
TACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA280.1559280503424848No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA270.1503591914016818No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGGTTAAAGGGGGGGTA260.14479033246087877No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTATAAAGGGAGCGCA250.13922147352007574No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTATTGGGCGTAAAGCGTGTGTA250.13922147352007574No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGAGCGTA250.13922147352007574No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAAGGGGGGCC240.13365261457927272No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGGTTAAAGGGAGCGTA240.13365261457927272No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGGGCGTA240.13365261457927272No Hit
TACGGAGGGTGCGAGCGTTAATCGGAATAACTGGGCGTAAAGGGCACGCA230.12808375563846966No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA230.12808375563846966No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTCATTGGGCGTAAAGCGCGCGTA230.12808375563846966No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA220.12251489669766664No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGGGTA220.12251489669766664No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGTGTA200.1113771788160606No Hit
TACGTAGGTTGCGAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA200.1113771788160606No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGGGTA200.1113771788160606No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGCA200.1113771788160606No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGGGGGAGCA190.10580831987525756No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGGGCGTA190.10580831987525756No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA190.10580831987525756No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGGGTAAAGGGGGCGCA180.10023946093445453No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)