FastQCFastQC Report
Wed 8 May 2024
EGAF00008390109

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008390109
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17792
Total Bases2.6 Mbp
Sequences flagged as poor quality0
Sequence length147-151
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA282015.849820143884893No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA178910.0550809352518No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA14858.346447841726619No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTA6803.8219424460431655No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGGGCGCA6353.569019784172662No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA5413.0406924460431655No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA5272.962005395683453No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGCA3922.2032374100719423No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGTA3592.0177607913669062No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGGGCGTA2091.174685251798561No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA1871.0510341726618706No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGCA1710.9611061151079136No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1590.893660071942446No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAGGGGGCGCA1550.8711780575539568No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA1520.85431654676259No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGCGTA1390.78125No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGTGTA1360.7643884892086331No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGGGCGCA1290.7250449640287769No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGCGCA1240.6969424460431655No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA1150.6463579136690647No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA1140.6407374100719424No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGGGGGCA860.48336330935251803No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA850.4777428057553957No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA770.43277877697841727No Hit
TACGTAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTAAAGCGTGCGCA760.427158273381295No Hit
TACGTAGGGTGCAAGCGTTATCCGGAATTATTGGGCGTAAAGGGCTCGTA700.39343525179856115No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAAGGGGGGCA670.37657374100719426No Hit
TACGTAGGGAGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGTGCGTA630.354091726618705No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATTATTGGGCGTAAAGAGGGAGCA630.354091726618705No Hit
TACGTATGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA570.3203687050359712No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAGGGGGGGCA530.297886690647482No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA510.28664568345323743No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGGTTAAAGGGGGCGTA460.2585431654676259No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAGGGAGCGCA440.24730215827338128No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAAGGGGCGCA440.24730215827338128No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGGGTA410.2304406474820144No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGCGCGCA400.22482014388489208No Hit
TACGGAGGATTCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGGGCGTA400.22482014388489208No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGTA390.21919964028776975No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGTA380.2135791366906475No Hit
TACGTAGGGGGCAAGCGTTATCCGGAATTACTGGGTGTAAAGGGTGCGTA370.2079586330935252No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGGGTAAAAGGGGCGCA360.20233812949640287No Hit
TACGTAGGGAGCGAGCGTTATCCGGATTTATTGGGTGTAAAGGGTGCGTA360.20233812949640287No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGTGCGTA360.20233812949640287No Hit
TACGTAGGTGGCAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGCGTGTA360.20233812949640287No Hit
TACGTAGGGAGCAAGCGTTATCCGGATTTATTGGGTGTAAAGGGGGCGTA360.20233812949640287No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGGGTAAAAGGGGGGCC350.19671762589928057No Hit
TACGGAGGATGCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA350.19671762589928057No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGCA350.19671762589928057No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGAGCGCA350.19671762589928057No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGCGCA340.19109712230215828No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGGGCA320.1798561151079137No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGGGTA310.17423561151079137No Hit
TACGTAGGGGGCTAGCGTTATCCGGAATTACTGGGCGTAAAGGGTGCGTA300.16861510791366907No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGGGTA300.16861510791366907No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA290.16299460431654678No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGGGCA280.15737410071942445No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGTGTA270.15175359712230216No Hit
AACGTAGGGTGCAAGCGTTGTCCGGAATTACTGGGGGTAAAGGGGGCGCA270.15175359712230216No Hit
TACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGCA270.15175359712230216No Hit
TACGTATGGAGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGGGCGTA260.14613309352517986No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAAGGGGGGTA240.13489208633093525No Hit
TACGGAGGATCCAAGCGTTATCCGGATTTATTGGGTTTAAAGGGGGCGTA240.13489208633093525No Hit
TACGTAGGGGGCTAGCGTTATCCGGATTTACTGGGCGTAAAGGGTGCGTA230.12927158273381295No Hit
AACGTAGGTCACAAGCGTTGTCCGGATTTACTGGGTGTAAAGGGAGCGCA230.12927158273381295No Hit
TACGTAGGGAGCGAGCGTTGTCCGGAATTACTGGGTGTAAAGGGGGCGTA220.12365107913669064No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGTGTAAAGGGAGTGTA220.12365107913669064No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGGGTAAAAGGGGGGCA200.11241007194244604No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGAGCGTA200.11241007194244604No Hit
TACGTAGGTGGCGAGCGTTATCCGGAATGATTGGGCGTAAAGGGTGCGTA190.10678956834532374No Hit
TACGTAGGTGGCAAGCGTTGTCCGGAATTATTGGGCGTAAAGCGCGCGCA190.10678956834532374No Hit
TACGGAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGTGCGTA190.10678956834532374No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGGGTA190.10678956834532374No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGGAAAGGGGGCGCA190.10678956834532374No Hit
TACGTAGGGGGCAAGCGTTATCCGGATTTACTGGGGGTAAAGGGGGCGCA180.10116906474820143No Hit
TACGTATGGTGCAAGCGTTATCCGGATTTACTGGGGGTAAAAGGGGGGCC180.10116906474820143No Hit
AACGTAGGTCACAAGCGTTGTCCGGAATTACTGGGTGTAAAGGGAGCGTA180.10116906474820143No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)