FastQCFastQC Report
Fri 10 May 2024
EGAF00008401544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008401544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22009497
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCAAAATCAGGCCACTGTCATTTCCGCTTTTCACTACCGTCGGATGACAT6841713.108526287538511No Hit
AGAGGCAGGAGGATCACTTAAGTCCAGAGATCGGAAGAGCACACGTCTGA6148742.793675839116178Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGCAAATCTTTGGACGCTGTCTGCAAGCTTGTCTATGTCATCCGACGG5878452.6708697613580172No Hit
AACATTGCTGGCCTACCTGTGAACAATCACTACCGTCGGATGACATAGGG5266552.3928534123246887No Hit
GCTATTGCTGAGCATCCCCGGAACCAGATCGGAAGAGCACACGTCTGAAC4220981.9177993935981363Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
CTCCCCTGCGGGAGGACTGCGAGATCGGAAGAGCACACGTCTGAACTCCA4141741.8817967534651063Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AACATTGCTGGAGCCTCCTCAGAAGATCGGAAGAGCACACGTCTGAACTC4094771.8604559658950863Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGGTGTGCTGGGAGCCCAAGGTCACTACCGTCGGATGACATAGGGACTCA3894941.769663341238557No Hit
TGCCATCATGTGGGGCCACGGAAAGATAGGAGAGATCGGAAGAGCACACG3859471.7535475708508923No Hit
CTCTGCATTCATTTTCACATATCCTTACTGCCAGCATTGAGAGATCGGAA3666951.6660762397250606No Hit
GTGACGATAGGGAGGGATGTGGCCTCTGCTATGTCATCCGACGGTAGTGA3623611.6463847401873837No Hit
CAGGCACTGCTTCAACTCCTGAGGCATCCTCGAGATCGGAAGAGCACACG3140601.4269294750352541No Hit
GTGACGATAGGGAGGTTTGCAAGGATCACATGAGATCGGAAGAGCACACG2568321.1669144460684404No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2373661.0784708073973703No Hit
GCTATTGCTGAGTATCTCGGGTTCCCAGATCGGAAGAGCACACGTCTGAA2339291.0628548212619306Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CTCTGCATTCATTTTCACACATCCTTACTGCCAGCATTGAGAGATCGGAA2194450.9970468657234647No Hit
CTTAGGGAAAAACGTGCCCAGATCGGAAGAGCACACGTCTGAACTCCAGT1981760.9004113088091018Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TGCCATTCCTGTGTGTGCCACTCCTCACTACCGTCGGATGACATACACTC1938430.880724352764627No Hit
TCAAAATCAGGCCACCGTCATTTCCGCTTTTCACTACCGTCGGATGACAT1807380.8211818743517856No Hit
GCAAGGGATGTGGCCTCTGCTATGTCATCCGACGGTAGTGAGCACTGGAG1549980.7042323593310651No Hit
ACGTGATGGTAAAGCCCAGTCAAGATCGGAAGAGCACACGTCTGAACTCC1520660.6909108372626599Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
ACTCCCTCAGAAGCTGCTCACTACCGTCGGATGACATAGGGACTCAGGAG1467230.6666349530841164No Hit
TGAGCAAATCTTTGGACCTGTCTATCCTCAGCTATCACTACCGTCGGATG1436290.6525773851169793No Hit
CTGCTCCTGTCTGTGCCTGCACAAGTCTTGGATGTTATGTCATCCGACGG1379810.6269157355118111No Hit
CACCCCAAGGTTCTGCCTCCCTCAGAAGATCGGAAGAGCACACGTCTGAA1373070.6238534210936306Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGCCCCACGGAAAGATAGGAGAGATCGGAAGAGCACACGTCTGAACTCCA1300450.5908585734603566Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
TGAGGTCAACTCGCTCCTTAGGCATCCTCGAGATCGGAAGAGCACACGTC1299470.590413311126556No Hit
CAGAACCTAGGGAAAGCCCCACGGAAAGATAGGAGAGATCGGAAGAGCAC1223430.5558645888181816No Hit
GCAGAATAGCATGCTGGATCAGATCGGAAGAGCACACGTCTGAACTCCAG1207770.5487494784637741Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)
AACATTGCTGGCCTACCTGTGAACAATCACTACCGTCGGATGACATATCT1181480.5368046348355894No Hit
TGAGCAAATCTTTGGACCTGTCTGCAAGCTTGTCTATGTCATCCGACGGT1077530.4895750230002984No Hit
TGAGCAAATCTTTCAGACCAGTCTATCCTCAGCTATCACTACCGTCGGAT1056080.4798292300819051No Hit
TGGTGTGCTGGGAGCCCAAGGTCACTACCGTCGGATGACATAAGTGACTG1037640.4714510286173282No Hit
GTGACGATAGGGAGGGTGTGGCCTCTGCTATGTCATCCGACGGTAGTGAG889430.404111915869772No Hit
TGCCATCATGTGGCCCCACGGAAAGATAGGAGAGATCGGAAGAGCACACG825060.37486545012818784No Hit
CTGTGACCTATGTACACCCCAGCCTCACTACCGTCGGATGACATACACTC813810.3697540202758836No Hit
GTGACGATAGGGATGTGGCCTCTGCTATGTCATCCGACGGTAGTGAGCAC808550.3673641428516063No Hit
AACATTGCTGGAGCCTCCCTCAGAAGATCGGAAGAGCACACGTCTGAACT791350.3595493345440834Illumina Multiplexing PCR Primer 2.01 (100% over 26bp)
CACCCCATCCTGTAGAAGTCCATATGTCATCCGACGGTAGTGAACAAGCT752930.34209323366181427No Hit
CAGGGCACACATGGATTATGTCATCCGACGGTAGTGATCATCTTAAGGAC747490.3396215733599No Hit
CACCCCATCCTGTAGAAGTCCATATGTCATCCGACGGTAGTGATTGTTCA730230.33177950409316487No Hit
GCCAGGAATGGAGCTCTTTCTATGTCATCCGACGGTAGTGAGCACTGGAG682360.3100298021349602No Hit
ACTCCCTCAGAAGCTGCTCACTACCGTCGGATGACATAGAAAGAGCTCCA677210.3076899031359054No Hit
ACCTCGGCCACTGTCATTTCCGCTTTTCACTACCGTCGGATGACATAGAA662430.30097462018327814No Hit
CACCCCATCCTGTAGAAGTCCATATGTCATCCGACGGTAGTGAGACATCA619020.281251316193187No Hit
AGAGGCGCTGATGGCAGAATTATGTCATCCGACGGTAGTGATGCGGGTGC594220.26998345305210747No Hit
GCAAGGGATGTGGCCTCTGCTATGTCATCCGACGGTAGTGAAGGAGAAGG549860.2498285172078217No Hit
ACGTGATGGTAAAGCCCAGGTCAAGATCGGAAGAGCACACGTCTGAACTC531990.24170929485576156Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
CGTCCAACCTTCCTTCTTAGCAAGATCGGAAGAGCACACGTCTGAACTCC529790.24070972635131097Illumina Multiplexing PCR Primer 2.01 (100% over 28bp)
TTCGCAGGAGGATCACTTAAGTCCAGAGATCGGAAGAGCACACGTCTGAA523300.23776099926318173Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
CAGGTCCCCAGGTGTAGGGAGAGATCGGAAGAGCACACGTCTGAACTCCA512950.23305848379906186Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
CACCCCATCCTGTAGAAGTCCATATGTCATCCGACGGTAGTGAACAAGAC500200.22726552996645039No Hit
ACTCCCTCAGAAGCTGCTCACTACCGTCGGATGACATAAGTGACTGTGAC499290.226852072085064No Hit
AACATTGCTGGAGCCTCCTCGAGATCGGAAGAGCACACGTCTGAACTCCA495180.2249846963790222Illumina Multiplexing PCR Primer 2.01 (100% over 29bp)
AGAGGCAGGAGGATCACTTAAGTCCAGTATGTCATCCGACGGTAGTGATC468520.21287174350236174No Hit
CTCGCATTCATTTTCACATATCCTTACTGCCAGCATTGAGAGATCGGAAG463560.21061817087414583No Hit
CTCCCCTGGGAGGTGGGCCTTCACTACCGTCGGATGACATAGGGACTCAG443070.2013085533031491No Hit
CACAAAAGTCTCTTCAGAGCCTGGGAGGTGGGCCTTCACTACCGTCGGAT439700.19977739609405884No Hit
GTGACGATAGGGAGGGTGTGTGTTTAGATCGGAAGAGCACACGTCTGAAC397230.18048118046496017Illumina Multiplexing PCR Primer 2.01 (100% over 25bp)
TGCCATTCCTGTGTGTGCCACTCCTCACTACCGTCGGATGACATAATTCC393620.17884097941902077No Hit
GTGACGATAGGGAGGGTGTGTTTAGATCGGAAGAGCACACGTCTGAACTC369680.16796385669331743Illumina Multiplexing PCR Primer 2.01 (100% over 27bp)
TGTGTGCACTGACTGCTGAGGGAGCAGTATGTCATCCGACGGTAGTGAAG363550.1651786953604619No Hit
TGCCATCATGTGGGGCCTGACAGCTAAGAGTGTATGTCATCCGACGGTAG354380.1610123120941837No Hit
CTGTGACCTCATCTGCCAGCCCAGTCAAGATCGGAAGAGCACACGTCTGA341800.1552965976460071Illumina Multiplexing PCR Primer 2.01 (100% over 23bp)
TGAGCAAATCTTTGGACCTGTCTATCCTCAGCTACCACTACCGTCGGATG341560.1551875538091579No Hit
GCAATTCCTGAAATCTCTGGGTTCCCAGATCGGAAGAGCACACGTCTGAA332460.15105297499529408Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
GGAAAATCTGGGAAATCCATCAAAAAATGAGGATAACGTGAAAAGCGCGC332000.15084397430799987No Hit
TGCCATTCCTGTGTGTGCCACTCCTCACTACCGTCGGATGACATATCTGC329970.1499216451879841No Hit
GGAAAATCTGGGAAATCCATCAAAAAATGAGGATAATGTGAAAAGCGCGC304680.1384311508800042No Hit
GTGACGATAGGGAGGGTGTGTGTTTCTGCAGACTGTCCACAGGCGCAGCA301800.13712262483781432No Hit
TGGTGTGCTGGGAGCCCAAGGTCACTACCGTCGGATGACATACTTACTTG295330.13418298473608917No Hit
GCTGAAACCCCAGACTCTGAAAACCAAAGAGCAAACTCTAGCCCGGGCAG294490.1338013313071171No Hit
TGCCATCATGTGGGGCCACGGCTTCCCTACTCTAGATCGGAAGAGCACAC293730.13345602582376145No Hit
ACGTGATGGTAAAGCCCAGGTGCCCAGCCACGCGTCTTCCTAGCTGGTAT275240.12505510689317434No Hit
CTCGCATTCATTTTCACACATCCTTACTGCCAGCATTGAGAGATCGGAAG272560.12383745071502543No Hit
GCAAGGGATGTGGCCTCTGCTATGTCATCCGACGGTAGTGACTCACCCCT272300.12371931989177218No Hit
ACTCCCTCAGAAGCTGCTCACTACCGTCGGATGACATACTTACTTGGATC270710.12299690447264652No Hit
TGAGGTCAACTCCTGAGGCATCCTCGAGATCGGAAGAGCACACGTCTGAA267370.1214793777431624Illumina Multiplexing PCR Primer 2.01 (100% over 24bp)
TGGTGTGCTGGGAGCCCAAGGTCACTACCGTCGGATGACATACCCTGGAT264240.12005726437092133No Hit
GTGACGATAGGGATGTGGCCTCTGCTATGTCATCCGACGGTAGTGAAAAG260220.11823078010369796No Hit
AACATTGCTGGCCTACCTGTGAACAATCACTACCGTCGGATGACATAACA252490.1147186598585147No Hit
CAGGGCACACATGGATTATGTCATCCGACGGTAGTGAATCTGCCTTGGAA252040.11451420266442255No Hit
ACTCCCTCAGAAGCTGCTCACTACCGTCGGATGACATACCCTGGATCAGA249370.11330108997947567No Hit
TCCGTTTTAGAATATTTCCATCATCCCTAAAAGATTGCTCAAGAGGCTGG247370.11239239133906603No Hit
TCCGTTTTAGAATATTTCCATCATCCCTAAAAGATTCCTCAAGAGGCTGG237760.10802609437189772No Hit
AGAGGCGCTGATGGCAGAATTATGTCATCCGACGGTAGTGAGCACCTTGG235760.1071173957314881No Hit
GTGACGATAGGGAGGGTGTGCAAGGATCACATGAGATCGGAAGAGCACAC233480.1060814792814211No Hit
TGACAACAATCTGTCTTTGCATTGACTGAAAACTATGCAATAATGTACAT232810.10577706523688388No Hit
CTCTGCATTATTTTCACATATCCTTACTGCCAGCATTGAGAGATCGGAAG230910.10491380152849472No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)