FastQCFastQC Report
Sat 11 May 2024
EGAF00008401725

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008401725
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25647161
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATACACAGTAAACATTCAAACACCTGGGGCCCTATGTCATCCGACGGTA7242932.8240669600818586No Hit
CGAGGATGCCTCAGGAGTTGAAGCAGTGCCTGCTTCAGCTTCCCGATTAC5741692.2387234204986663No Hit
CTGGACTTAAGTGATCCTCCTGCCTCTCTTCAGCTTCCCGATTACGGATC5006731.952157589684098No Hit
CTCAATGCTGGCAGTAAGGATATGTGAAAATGAATGCAGAGCTTCAGCTT4124111.6080181350286684No Hit
GGTTCCGGGGATGCTCAGCAATAGCCTTCAGCTTCCCGATTACGGATCTC3379161.3175571362459961No Hit
CATGTGATCCTTGCAAACCTCCCTATCGTCACCTTCAGCTTCCCGATTAC3290441.2829646135102437No Hit
CCACCCTCCTGAGTCCCTATGTCATCCGACGGTAGTGACCTTGGGCTCCC3088251.2041293771267705No Hit
GGGAACCCGAGATACTCAGCAATAGCCTTCAGCTTCCCGATTACGGATCT3070251.1971110564635206No Hit
GCCAGGAATGGAGCTCTTTCTATGTCATCCGACGGTAGTGAAAAGCGGAA3012731.1746836228774016No Hit
CTCCTATCTTTCCGTGGCCCCACATGATGGCACTTCAGCTTCCCGATTAC2599831.0136911449965165No Hit
TCTGAGGAGGCTCCAGCAATGTTCTTCAGCTTCCCGATTACGGATCTCGT2466810.9618257552950987No Hit
CTCAATGCTGGCAGTAAGGATGTGTGAAAATGAATGCAGAGCTTCAGCTT2276360.8875680236108784No Hit
GGGCACGTTTTTCCCTAAGCTTCAGCTTCCCGATTACGGATCTCGTATGT2132330.8314097611037728No Hit
ACCTCCCTTGGTCCTTAAGATGATCACTACCGTCGGATGACATAGACAAG1770600.6903688092416934No Hit
TCAGCTTCCCGATTACGGATCTCGTATGTGTAGATCTCGGTGGTCGCCGT1700490.6630324502583347Illumina Single End PCR Primer 1 (95% over 24bp)
GGACACTTTGAGGTTGCTCCAGTGCTCACTACCGTCGGATGACATAGCAG1582910.6171872200591715No Hit
GCCAGGAATGGAGCTCTTTCTATGTCATCCGACGGTAGTGAGCAGCTTCT1572560.6131516856778028No Hit
TGACTGGGCTTTACCATCACGTCTTCAGCTTCCCGATTACGGATCTCGTA1294880.5048823922460657No Hit
GGCCGTTTCTATGCACCCGCATCACTACCGTCGGATGACATAGCAGAGGC1280230.4991702590395873No Hit
CGCAGTCCGCATGGTCACAGTCACTTATGTCATCCGACGGTAGTGACCTT994050.38758675862798225No Hit
GGCCGTTTCTATGCACCCGCATCACTACCGTCGGATGACATAATTCTGCC862440.3362711373785192No Hit
GGTGCCGGGATTATGAAATGTCTAACCCAAGCTTGTTCACTACCGTCGGA834090.32521728233390046No Hit
ACCTCCCTTGGTCCTTAAGATGATCACTACCGTCGGATGACATAATCCAT735350.28671789442893897No Hit
CGGGAAAACCTTGGACATAGAACTGGCAGCCACTTGGGATTGATTCACTG687200.26794388665474517No Hit
GAATGAAGCACAATCATTAGCTGTAGACAACGTAGCCTAATGGAGCTGGA631980.24641323848670812No Hit
TCTGAGGGAGCAGAGCCATGGGGTGCTTCAGCTTCCCGATTACGGATCTC627370.24461576858350909No Hit
TGCTAAGAAGGAAGGTTGGACGCTTCAGCTTCCCGATTACGGATCTCGTA625430.24385934957869218No Hit
CGGGAAAACCTTGGACATAGAACTGGCAGCCTCTTGGGATTGATTCACTG591010.2304387608437441No Hit
AAACACACACACCCTCCCTATCGTCACCTTCAGCTTCCCGATTACGGATC576940.22495277352530363No Hit
ACCTCCCTTGGTCCTTAAGATGATCACTACCGTCGGATGACATACTGGAC552570.21545074716067014No Hit
CTCAATGCTGGCAGTAAGGATATGTGAAAATGAATGCGAGCTTCAGCTTC540970.21092782939990903No Hit
CTGGACTTAAGTGATCCTCCTGCGAACTTCAGCTTCCCGATTACGGATCT500850.19528477245493175No Hit
TGGGCTTTTGATTCACTGTTTAAAGGCCACTTTCACTACCGTCGGATGAC488600.19050841533688662No Hit
CAGGGGAGACCTGTCTATGTCATCCGACGGTAGTGAGCACCTTGGACTCT459980.17934928548231907No Hit
CTGGACTTAAGTGATTCTCCTGAATTCCAAGGCAGATTCACTACCGTCGG457640.17843690379609659No Hit
CCGCCCTTTGCTTCGAGCGTGAGTTATGTCATCCGACGGTAGTGACCTTG429380.16741814035479405No Hit
AGAGTAGGGAAGCCGTGGCCCCACATGATGGCACTTCAGCTTCCCGATTA425050.1657298443285789No Hit
ACACAGAATAAGTTTTGTAGTCTGTCCAGAAAATACCATGGGGTAAGCCT419930.16373352200658778No Hit
CTGGACTTAAGTGATCTCCTGAATTCCAAGGCAGATTCACTACCGTCGGA416070.16222848213102417No Hit
ACACAGAATAAGTTTTGTAGTCTGTCCAGAAAATACCATGGAGTAAGCCT402960.15711680524795707No Hit
GCCAGGAATGGAGCTCTTTCTATGTCATCCGACGGTAGTGAAGGCCCACC369450.14405103161320662No Hit
CTGGCCACTGTCATTTCCGCTTTTCACTACCGTCGGATGACATAGCAGAG319690.12464927404635547No Hit
AACTCGTCAAAGGAAGTTTCACTGTAGGGTATTTTCATAGGATGCACAAC318950.12436074308575518No Hit
AACTCGTCAAAGGAAGTTTCACTGTAGGGTATTTTATAGGATGCACAACT309320.12060594153091642No Hit
AAACACACACAGCTTCCTGCCACGTCCTACAGTATGTCATCCGACGGTAG293550.11445711281650238No Hit
TGCTAAGAAGGAAGGTTGGACGTATTTCCACTACTTTGAATTGTCTCCCT293190.11431674640323738No Hit
CTCAATGCTGGCAGTAAGGATGCCGTCATTGTTAAGTGTGGATCTTTAGC292830.11417637998997238No Hit
CCCAAAGGTGGAAATCATTAAGAAAAACACTGATGGACTAAATATATTAT291780.1137669779512828No Hit
CTCAATGCTGGCAGTAAGGATGTGTGAAAATGAATGCGAGCTTCAGCTTC283880.11068671499352306No Hit
CTCAATGCTGGCAGTAAGGATGCCTTCATTGTTAAGTGTGGATCTTTAGC279840.10911149191132695No Hit
TGGGCTTTTGGTACACCTGCCAGGTGGTGCTAACCAAACTGAGCTTCATG276170.10768053430943098No Hit
ACCTCATTGGAAGGACCTAGCCATTGGAGATTAACTGATGGAAATATTTT268470.104678252692374No Hit
TGGGCTTTTGGTACACCTGCCAGGTGGTGCTGACCAAACTGAGCTTCATG264960.10330968016304028No Hit
CTCAATGCTGGCAGTAAGGATATGTGAAAATAATGCAGAGCTTCAGCTTC261700.10203858430958498No Hit
ACCTCATTGGAAGGACCTAGCCATTGGAGATGAACTGATGGAAATATTTT257930.10056863603733764No Hit
CCCAAAGGTGGAAATCATTAAGAAAAACACTGATGAACTAAATATATTAT257360.10034638921633471No Hit
TGGGCTTTTGGTACACCTGCCTCTCTTCAGCTTCCCGATTACGGATCTCG256810.10013194052940207No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)