FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402034

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402034
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8344497
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTTAGCTGTGTAGATC5509576.602638840903173Illumina Single End Sequencing Primer (100% over 33bp)
AAGCAGAACACATCTCGTATGCCGTCTTCTGCTTAGATCGGAAGAGCGTC624020.7478221874847579Illumina PCR Primer Index 9 (96% over 26bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTTAGCTGTGTAGATCT335880.40251677243098055Illumina Single End Sequencing Primer (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)