FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402043

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402043
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20752148
Total Bases3.1 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTTCGAGGTGTAGATC726222034.99502798457297Illumina Single End PCR Primer 1 (97% over 35bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTTCGAGGTGTAGATCT1812180.8732493619455682Illumina Single End PCR Primer 1 (97% over 34bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTTCGAGGTGTAGAT1388280.6689813507498116Illumina Single End PCR Primer 1 (97% over 35bp)
CTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTTCGAGGTGTAGA386200.1861012170884672Illumina Single End PCR Primer 1 (97% over 35bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTTCGAGGTGTAGAT350140.16872470261873612Illumina Single End PCR Primer 1 (97% over 35bp)
NGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGTTCGAGGTGTAGATC327080.15761259991013943Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)