FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402059

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402059
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5059846
Total Bases758.9 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAGATACGGTGTAGATC4089078.08141196392143Illumina Single End Sequencing Primer (100% over 33bp)
AAGCAGAAGACGGCACACGTCTGAACTCCAGTCACGTCAGTCAATCTCGT279400.552190718847965TruSeq Adapter, Index 18 (96% over 28bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAGATACGGTGTAGATCT117710.23263553871007142Illumina Single End Sequencing Primer (100% over 32bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGAGATACGGTGTAGAT59720.11802730755046695Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)