FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402074

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402074
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3574883
Total Bases536.2 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCACGTAAGTGTAGATC60349816.88161542629507Illumina Single End Sequencing Primer (100% over 33bp)
AAGCAGAAGACGGCACACGTCTGAACTCCAGTCACCCTTCCATATCTCGT288860.8080264445018202TruSeq Adapter, Index 16 (96% over 29bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCACGTAAGTGTAGATCT164990.4615255939844745Illumina Single End Sequencing Primer (100% over 32bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCACGTAAGTGTAGAT55800.15608902445198905Illumina Single End Sequencing Primer (100% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)