FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402075

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402075
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10210504
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGTACGTGTAGATC133063913.03205992573922Illumina Single End PCR Primer 1 (97% over 37bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGTACGTGTAGATCT292410.2863815537411278Illumina Single End PCR Primer 1 (97% over 36bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGTACGTGTAGAT186410.1825668938575412Illumina Single End PCR Primer 1 (97% over 37bp)
CGGTGGTCGCCGTATCATTAGAACGGAAGAGCGTCGTGTAGGGAAAGAGT140680.1377796825700279Illumina Single End PCR Primer 1 (96% over 31bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT121630.11912242529849652Illumina Single End PCR Primer 1 (100% over 31bp)
CGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGGTGTAC112150.1098378689240022Illumina Single End PCR Primer 1 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)