FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402086

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402086
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6369979
Total Bases955.4 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC43

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTA278182343.67083470761835TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGTAT658031.033017534280725TruSeq Adapter, Index 7 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATATCGTA503950.7911329064036162TruSeq Adapter, Index 7 (97% over 35bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATCCGTATCTCGT137650.21609176419576892TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)