FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10255968
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACCTTGGGTGTAGATC192441918.763894349124335Illumina Single End PCR Primer 1 (97% over 34bp)
AAGCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACCTTGGGT3244703.1637189195598117Illumina Single End PCR Primer 1 (97% over 34bp)
CGGTGGTCGCCGTATCATTAGAACGGAAGAGCGTCGTGTAGGGAAAGAGT1221481.1909943556766167Illumina Single End PCR Primer 1 (96% over 31bp)
AAGCAGAAGACGGCATACGAGATTCAAGGACGTGACTGGAGTTCAGACGA1108251.0805903450556789Illumina PCR Primer Index 8 (96% over 28bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAACCTTGGGTGTAGATCT726170.7080462809556348Illumina Single End PCR Primer 1 (96% over 33bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT713270.695468238590448Illumina Single End PCR Primer 1 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT416580.40618301461159007Illumina Single End PCR Primer 1 (100% over 31bp)
AAGCAGAAGACGGCACACGTCTGAACTCCAGTCACGTCCTTGAATCTCGT330860.32260241061594574TruSeq Adapter, Index 18 (96% over 27bp)
AAGCAGAAGACGGCATACGAGATTCAAGGACGTGACTGGTGTTCAGACGA232540.22673627686825856Illumina PCR Primer Index 8 (96% over 28bp)
CGGTGGTCGCCGTATCATTAGAAAGGAAGAGCGTCGTGTAGGGAAAGAGT204830.19971786183420231Illumina Single End PCR Primer 1 (100% over 26bp)
AAGCAGAAGACGGCATACGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT124400.12129523025032839Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)