FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402119

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402119
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5059846
Total Bases758.9 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTACATCTCGTA3972117.850258683762313TruSeq Adapter, Index 2 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATTGACTGACGTGACTGGAGTTCAGACGT286180.5655903361485706RNA PCR Primer, Index 32 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTACATCTCGTAT113570.2244534715088167TruSeq Adapter, Index 2 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTACATATCGTA79930.1579692346367854TruSeq Adapter, Index 2 (97% over 36bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGGTACATCTCGT59180.11696008139378154TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)