FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5253715
Total Bases788 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGAAGGTGTAGATC331191263.039430193682Illumina Single End Sequencing Primer (100% over 33bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGAAGGTGTAGATCT1896323.6094839556390097Illumina Single End Sequencing Primer (100% over 32bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGAAGGTGTAGAT291770.5553593980640366Illumina Single End Sequencing Primer (100% over 33bp)
AAGCAGAAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGAAGGT228610.43513970590334644Illumina Single End Sequencing Primer (100% over 33bp)
NGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGAAGGTGTAGATC151590.28853868167572855Illumina Single End PCR Primer 1 (96% over 33bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGAAGGTGTAGAT87900.16731017955865518Illumina Single End Sequencing Primer (100% over 33bp)
AGATCGGAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGAAGGTGTAGATCT71940.13693167596643518Illumina Single End Sequencing Primer (100% over 25bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGCGTCAGTGAAGGTGTAGATC66400.1263867567997122Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTCAGTGAAGGTGTAGATC65010.12374100993297124Illumina Single End PCR Primer 1 (96% over 33bp)
CTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGAAGGTGTAGA61810.1176500818944309Illumina Single End Sequencing Primer (100% over 33bp)
AGATCGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGTGAAGGTGTAGATCT54910.10451651831132826Illumina Single End PCR Primer 1 (96% over 28bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)