FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5504028
Total Bases825.6 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTA2542594.619507749597204TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTAT108380.19691033548521192TruSeq Adapter, Index 22 (97% over 38bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGT76330.13868025380684837TruSeq Adapter, Index 22 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)