FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8344497
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCTCGTA5446296.526804431711103TruSeq Adapter, Index 20 (97% over 37bp)
AAGCAGAAGACGGCATACGAGATGTGTTCTGCTTAGATCGGAAGAGCACA493670.5916114536322561RNA PCR Primer, Index 39 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATCTCGTAT328340.3934808772775639TruSeq Adapter, Index 20 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGTTACATATCGTA122980.14737856577814096TruSeq Adapter, Index 20 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)