FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17209906
Total Bases2.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTATCTCGTA12763907.416600648486982TruSeq Adapter, Index 6 (97% over 36bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTATCTCGT399750.23227901419101302TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTATATCGTA286490.1664680794886387TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACGAACTATCTCGTAT277950.16150582112418277TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)