FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences284365
Total Bases42.6 Mbp
Sequences flagged as poor quality0
Sequence length150
%GC37

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATATCGTAT12850.45188402229528957TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGAAATCGTAT11400.40089321822305835TruSeq Adapter, Index 22 (97% over 37bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACAATCTCGTA4590.16141226944244194TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAATACGATCTCGTAT3940.13855432278937282TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGAATACGAAATCGTAT3260.11464139398308512TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)