FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10527594
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGGAATATCTCGTA2395472.275420195725633TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGAGGAATATCTCGTAT234450.22270045748344777TruSeq Adapter, Index 2 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)