FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45740244
Total Bases6.8 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGATGACGTGTAGATC803124417.558375945698934Illumina Single End PCR Primer 1 (97% over 34bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGATGACGTGTAGAT1915580.4187953173140047Illumina Single End PCR Primer 1 (97% over 34bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGATGACGTGTAGATCT934440.20429274491845736Illumina Single End PCR Primer 1 (96% over 33bp)
AATGATACGGTCTTGTGTAGATCTCGGTGGTCGCCGTATCATTAAAAAAA540090.11807763858889778Illumina Single End PCR Primer 1 (96% over 30bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTACGATGACGTGTAGAT487400.10655824223412538Illumina Single End PCR Primer 1 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)