FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402273

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402273
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10210504
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCTCGTA123132112.059355737973366TruSeq Adapter, Index 6 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATATCGTA829780.8126729101717212TruSeq Adapter, Index 6 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCTCGTAT276850.2711423451770843TruSeq Adapter, Index 6 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT260160.25479643316333844Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCATGATCTCGT179440.1757405902784035TruSeq Adapter, Index 6 (97% over 36bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT122220.11970026161294291Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGTATGCCGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCTCAT112180.1098672504315164TruSeq Adapter, Index 6 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)