FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402274

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402274
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10255968
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGTA185309718.068474862636076TruSeq Adapter, Index 15 (97% over 36bp)
TCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCA3160723.081834888720402TruSeq Adapter, Index 15 (97% over 36bp)
AATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGT2213482.1582360631390425Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGTGGTCGCCGTATCATTAGATCGGAAGAGCACACGTCTGAACTCCAGT1733251.6899916224387597Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
AATGATACGGCGACCACCGAGATCTACACTCGTCTGAACTCCAGTCACGT1081531.0545372216449973Illumina Single End PCR Primer 1 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATCTCGTAT698760.6813203785347224TruSeq Adapter, Index 15 (97% over 36bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGATAGCATATCGTA457820.44639374849843527TruSeq Adapter, Index 15 (97% over 36bp)
AAGCAGAAGACGGCATACGAGATTCAAGGACGTGACTGGAGTTCAGACGT452860.4415575399611231Illumina PCR Primer Index 8 (96% over 28bp)
AATGATACGGCGACCACCGAGATCTACACTCGTCTGAACACCAGTCACGT216710.2113013613146999Illumina Single End PCR Primer 1 (96% over 33bp)
CGGTGGTCGCCGTATCATAGATCGGAAGAGCACACGTCTGAACTCCAGTC201700.1966659802370678Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ATGATACGGCGACCACCGAGATCTACACTCGTCTGAACTCCAGTCACGTC172090.16779498531976697Illumina Single End PCR Primer 1 (96% over 32bp)
GATACGGCGACCACCGAGATCTACACTCGTCTGAACTCCAGTCACGTCCT134870.13150392044904977Illumina Single End PCR Primer 1 (96% over 30bp)
CGTATGCCGTCTTCTGCTTAGATCGGAAGAGCACACGTCTGAACTCCAGT127160.12398634629125208Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
ATGATACGGCGACCACCGAGATCGGAAGAGCACACGTCTGAACTCCAGTC116880.11396291408085517Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CGGTGGTCGCCAGATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGAT103450.10086809943244752TruSeq Adapter, Index 15 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)