FastQCFastQC Report
Wed 10 Jul 2024
EGAF00008402333

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00008402333
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24385500
Total Bases3.6 Gbp
Sequences flagged as poor quality0
Sequence length150
%GC42

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGTAGCTGTGTAGATC770500831.59667835393984Illumina Single End PCR Primer 1 (97% over 35bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGTAGCTGTGTAGAT1749780.7175493633511718Illumina Single End PCR Primer 1 (97% over 35bp)
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGTAGCTGTGTAGATCT1440400.59067888704353Illumina Single End PCR Primer 1 (97% over 34bp)
CTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGTAGCTGTGTAGA589740.24184043796518423Illumina Single End PCR Primer 1 (97% over 35bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGTAGCTGTGTAGAT398800.16353980849275185Illumina Single End PCR Primer 1 (97% over 35bp)
NGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGGTAGCTGTGTAGATC344300.14119046154477047Illumina Single End PCR Primer 1 (96% over 33bp)
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGAGTTGGTAGCTGTGTAGATC284600.11670869984211929Illumina Single End PCR Primer 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)